Milk clotting enzyme (M CE) produced by: Rhizomucor miehei was purified and characterized.The enzyme was purified 220.29-fold with specific activity about 14444.2 U/mg protein byultrafiltration, ammonium sulfate fractionation, Sephacryl S-300 chromatography. The maximumenzyme activity was at 65°C.The milk clotting activity was decreased steadily as pH is increased and indicated maximumactivity ...

Following a veterinary and behavioral survey of chimpanzees from a natural population in Uganda, leaf samples of Trichilia rubescens were collected because of the unusual method of ingestion observed. The methanolic crude extract of T. rubescens leaves exhibited significant antimalarial activity in vitro. Bioassay-directed fractionation provided two new limonoids, trichirubines A and B. A great...

An EtOH extract of fruits of Piper longum was found to exhibit a potent inhibitory effect against alpha-melanocyte-stimulating hormone (alpha-MSH)-induced melanin production in B16 mouse melanoma cells. Bioassay-directed fractionation led to the isolation of prenylated phenolic compounds bakuchiol, bavachin, and isobavachalcone. These compounds and the crude extract of the fruits of P. longum m...

Fractionation of petroleum ether partitioned ethanol extract and crude petroleum ether extract of the leaves of Murraya koenigii Linn (Rutaceae) led to the isolation of 5,8-dimethyl furanocoumarin (1) and 1-al, 3[6’, 6’ dimethyl 5-hexene] carbazole (2) and β-sitosterol (3). Their structures were elucidated by spectroscopic methods such as UV, IR, NMR and LCMS. All the compounds were isolated fo...

this study was carried out to determine the effects of gamma irradiation on dry matter (dm) and on crude protein (cp) degradation of rapeseed meal, using nylon bags and sds-page techniques. ruminal nylon bags of either untreated or irradiated rapeseed meal at doses of 25, 50 and 75 kgy were suspended in the rumen of four non-lactating holstein cows for up to 48 h. intestinal crude protein diges...

the cornell net carbohydrate and protein system (cncps) model has recently been increasingly employed in preparation of balanced feed ration of dairy cattle in iran. however, a lack of appropriate characterization of the feeds has restricted the model's application. this study was conducted to evaluate the protein contents of some feedstuffs (wheat bran, sugar beet pulp, soybean meal, cott...

As part of our search for new antimalarial drugs in South Pacific marine sponges, we have looked for inhibitors of Pfnek-1, a specific protein kinase of Plasmodium falciparum. On the basis of promising activity in a preliminary screening, the ethanolic crude extract of a new species of Pseudoceratina collected in Vanuatu was selected for further investigation. A bioassay-guided fractionation le...

We measured rates of protein synthesis in vivo in subcellular fractions (soluble, myofibrillar and stromal fractions) of the heart and the gastrocnemius from rats after fasting or under hypoxic conditions (i.e. atmospheres containing 5% or 10% O2). Such interventions are known to inhibit protein synthesis under some circumstances. The recovery of tissue protein after fractionation was 80-100%. ...

A procedure for the isolation of pure tetanus toxin in a lethal monomeric form was developed based on the extraction of whole cells and chromatographic techniques. A crude extract of toxin was obtained by hypertonic extraction of cells from a 72-hr culture of Clostridium tetani Massachusetts strain. The extract was precipitated with ammonium sulfate and further purified by sequential use of ion...

Shiga toxin has been purified in milligram quantities to near homogeneity from cell lysates of Shigella dysenteriae 1 strain 3818-0. Purification involved an initial ultracentrifugation, ammonium sulfate fractionation, chromatography on DEAE-cellulose and carboxymethyl cellulose, gel filtration, and preparative isoelectric focusing in sucrose gradients. The purified toxin was resolved by discon...