Cost-effective production of lignocellulosic biofuel requires efficient breakdown of cell walls present in plant biomass to retrieve the wall polysaccharides for fermentation. In-depth knowledge of plant cell wall composition is therefore essential for improving the fuel production process. The precise spatial three-dimensional (3D) organization of cellulose, hemicellulose, pectin and lignin wi...

Although electron cryo-microscopy (cryo-EM) single-particle analysis has become an important tool for structural biology of large and flexible macro-molecular assemblies, the technique has not yet reached its full potential. Besides fundamental limits imposed by radiation damage, poor detectors and beam-induced sample movement have been shown to degrade attainable resolutions. A new generation ...

Accurate determination of micrograph focuses is essential for averaging multiple images to reach high-resolution 3-D reconstructions in electron cryo-microscopy (cryo-EM). Current methods use iterative fitting of focus-dependent simulated power spectra to the power spectra of experimental images, with the fitting performed independently for different images. Here we have developed a novel graph...

The Tera-eV Energy Superconducting Linear Accelerator (TESLA) is a 32 km long superconducting linear electron/positron collider of 500 GeV centre of mass energy (upgradeable to 800 GeV), presently in the planning phase at DESY [1,2]. About 21000 superconducting RF 9-cell cavities have to be cooled with superfluid helium at 2 K. The cavities are assembled in groups of 12 into about 16 m long cry...

To truly understand bioenergetic processes such as ATP synthesis, membrane-bound substrate transport or flagellar rotation, systems need to be analysed in a cellular context. Cryo-ET (cryo-electron tomography) is an essential part of this process, as it is currently the only technique which can directly determine the spatial organization of proteins at the level of both the cell and the individ...

the aim of this research work was to investigate on the ball filling estimation of miduk semi-autogenous (sag) mill via an utilized method. miduk copper concentrator is located in kerman province, iran, and its size reduction stage includes one gyratory crusher which feeds one sag mill (9.75 dia.(m)*3.88 length(m)) following two parallel ball mills (5dia.(m)*7lenght(m)). after sag mill, a tromm...

Recent advances in electron microscopy and image analysis techniques have resulted in the development of tomography, which makes possible the study of structures neither accessible to X-ray crystallography nor nuclear magnetic resonance. However, the use of tomography to study biological structures, ranging from 100 to 500 nm, requires developments in sample preparation and image analysis. Inde...

Abstract Cryo-electron microscopy (cryo-EM) maps usually show heterogeneous distributions of B -factors and electron density occupancies are typically -factor sharpened to improve their contrast interpretability at high-resolutions. However, ‘over-sharpening’ due the application a single global can distort processed causing connected densities appear broken disconnected. This issue limits cryo-...

A new, ligand- and solvent-free method for the Huisgen 1,3-dipolar cycloaddition (click reaction) was developed using a planetary ball mill. Besides various alkynes and azides, a propargyl functionalized polymer was converted by mill clicking. Moreover, it was possible to carry out a click polymerization in a ball mill.