Our objective is to describe a successful live birth from oocyte vitrification followed by thaw, fertilization, blastocyst culture, trophectoderm biopsy, vitrification, and subsequent thaw. Fifteen mature oocytes were frozen from a patient with uterine factor infertility. Thirteen oocytes survived the thaw, and five underwent trophectoderm biopsy and were refrozen. Three euploid embryos were ob...

In cryopreservation of mammalian germ cells, unfertilized oocytes are one of the most available stages because these cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, it has been generally reported that the fertility and developmental ability of the oocytes are reduced by cryopreservati...

Abstract Study question Is the human egg spindle integrity impaired by vitrification and thawing procedures? Summary answer Cryoprotective-caused dehydration stabilizes MII spindle, but re-entry of water during induces depolymerization microtubules, thus promoting instability division apparatus. What is known already Freezing sperm preimplantation embryos have become routine IVF procedures with...

objective: vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. however, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. this study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mo...

Heterogeneity in morphology, physiology and cellular chemistry of plant tissues can compromise successful cryoprotection and cryopreservation. Cryoprotection is a function of exposure time × temperature × permeability for the chosen protectant and diffusion pathway length, as determined by specimen geometry, to provide sufficient dehydration whilst avoiding excessive chemical toxicity. We have ...

Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of...

In mammals, unfertilized oocytes are one of the most available stages for cryopreservation because the cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, it has generally been reported that the fertility and developmental ability of the oocytes are reduced by cryopreservation. C57BL/6J m...

Background: Embryo vitrification is a key instrument in assisted reproductive technologies (ARTs). However, thereis increasing concern that vitrification adversely affects embryo development. This study intends to assess the effectof vitrification on developmental competence, in addition to expressions of long non-coding RNA (lncRNA) gene traplocus 2 (Gtl2) and its reciprocal ...

Oocyte cryopreservation has encompassed various technical difficulties and thus remained a challenge to many cryobiologists. The effect of three widely used cryoprotectants and two cryopreservation techniques on the post thaw recovery rate of morphologically normal sheep oocyte, their fertilizability and developmental competence were analyzed. Ethylene glycol, propylene glycol and Dimethyl Sulf...

Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...