Helper Virus Independent Transcription and Multimerization of A 1 Satellite RNA Associated with Cucumber Mosaic Virus 2 3 Soon Ho Choi#, Jang-Kyun Seo#, Sun-Jung Kwon# and A. L. N. Rao* 4 Department of Plant Pathology & Microbiology, University of California, Riverside, 5 California 92521-0122 6 . 7 8 9 # These authors contributed equally to this work 10 11 12 Running title: Helper virus indepe...

The replication-associated proteins encoded by Cucumber mosaic virus (CMV), the 1a and 2a proteins, were detected by immunogold labeling in two host species of this virus, tobacco (Nicotiana tabacum) and cucumber (Cucumis sativus). In both hosts, the 1a and 2a proteins colocalized predominantly to the vacuolar membranes, the tonoplast. While plus-strand CMV RNAs were found distributed throughou...

Infectious material was formed at an early stage, and migrated into the mesophyll from the epidermis of tobacco leaves (Nicotiana tabacum cv. Samsun NN) during the period of 1 to 3 hours after inoculation with tobacco mosaic virus (TMV). The activity of membrane-bound Mg(2+)-activated ATPase from the mesophyll was stimulated two to four times within 30 minutes after inoculation with 1.0 microgr...

To date, there have been no reports of Dalmatian toadflax serving as a host for cucumber mosaic virus (CMV). Infestations of Dalmatian toadflax may serve as a reservoir of CMV, thereby facilitating aphid transmission of CMV to both agricultural crops and native plants. The goal of this study was to determine whether Dalmatian toadflax is a host for CMV. Dalmatian toadflax seedlings were randoml...

The strategy for mass cloning of plants by micro­ propagation depends on disease-indexing the donor plant, and eliminating any diseases found, before setting up the explant or meristem cultures. Details of tissue culture media have been published for most commercially important species [1]. Fungal, bacterial and mycoplasmal pathogens can be successfully elim­ inated by the addition of the appro...

Cucumber mosaic virus (CMV) is the type species of the Cucumovirus genus in the Family Bromoviridae. The virus is distributed worldwide, has a very broad host range infecting over 1000 species in more than 85 plant families, and is transmitted by more than 80 aphid species in 30 genera in a non-persistent manner. The viral genome is positive-sense, single-stranded RNA divided among three segmen...

The 5'-terminal sequences of the virion protein mRNAs of ononis yellow mosaic and kennedya yellow mosaic tymoviruses were determined, and also the positions in the genomes of the transcription initiation sites of those mRNAs. Comparisons of the available genomic sequences of tymoviruses revealed two conserved regions, one at the initiation site and another longer sequence of sixteen nucleotides...