Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of both polarities...

The complete genome sequences of two Slovak Zucchini yellow mosaic virus isolates (ZYMV-H and ZYMV-SE04T) were determined. These isolates differ significantly in their pathogenicity, producing either severe or very mild symptoms on susceptible cucurbit hosts. The viral genome of both isolates consisted of 9593 nucleotides in size, and contained an open reading frame encoding a single polyprotei...

watermelon mosaic virus is the most prevalent potyvirus infecting cucurbits in iran. in order to determine the distribution and frequency of the virus, 326 watermelon, melon, cucumber, winter and summer squash samples were collected from 22 villages around urmia during summer 2010 and tested by das-elisa with a polyclonal antibody. ninety four samples were detected as infected by elisa. to dete...