The aim of this study was to optimize candidate antigen proteins for serological screening of Chlamydia trachomatis infection. C. trachomatis positive serum and swabs of genital secretions were collected from 50 patients in the Tianjin Medical University General Hospital, as well as from 30 patients negative for C. trachomatis. Samples were assessed by colloidal gold assay in a sexually transmi...

Chickens were experimentally infected with Salmonella thompson (serogroup C, paratyphoid) and Salmonella pullorum (serogroup D). Five serological methods and one cultural method were used in detecting the infections. The microantiglobulin test was superior to all other methods for detection of paratyphoid (S. THOMPSON) infection and was followed in efficacy by the microagglutination test, rapid...

The diagnosis of invasive candidiasis (IC) and cryptococcosis is often complicated by slow and insensitive culture-based methods. Such delay results in poor outcomes due to the lack of timely therapeutic interventions. Advances in serological, biochemical, molecular and proteomic approaches have made a favorable impact on this process, improving the timeliness and accuracy of diagnosis with res...

Salmonella prevalence was assessed in six herds of sows by serological ELISA test and faecal culture. Blood and faecal samples were collected, prior to weaning of piglets, from 45 sows housed in separated pens in each group of a herd. Increased levels of specific antibodies were found in all six herds. Serologically positive samples averaged 41 .85%. Faecal shedding of Salmonella from the carri...

AIMS To compare the culture and PCR methods for detection of Brucella melitensis in blood and lymphoid tissue samples obtained from slaughtered sheep (n = 162) testing positive/negative in serological tests (Rose Bengal test and serum agglutination test). METHODS AND RESULTS Of 162 sheep examined, 45 were positive and 117 negative in serological tests. A PCR assay based on a pair of Br. melit...

Abstract Objective The aim of this study was to investigate the genetic relatedness and antimicrobial resistance among Shigella species isolated from food stool samples. Using cross sectional method, spp. were clinical samples using culture-based, biochemical serological methods. Antimicrobial susceptibility isolates evaluated disk diffusion RAPD-PCR methods respectively. Results prevalence 4.8...

Brucellosis is an infectious disease that affects many organ systems. Osteoarticular involvement, especially spondylitis, is the most common complication of brucellosis, but psoas abscess is very rare. Serological tests at diagnosis of the disease are very valuable and most widely used. Herein, we report a case of psoas abscess secondary to brucellar spondylitis. In this case, interestingly, th...

In order to laboratory confirm the first suspected West Nile fever case in 2013 in northern Greece, a combination of serological molecular and culture methods were applied. It was shown that the causative West Nile virus strain belonged to lineage 2, and possessed the amino acid substitution H249P in the NS3 protein, as in previous years. The significance of this specific strain in Europe remai...