Two cyclic nucleotide phosphodiesterase (PDE) activities were identified in pig aortic endothelial cells, a cyclic GMP-stimulated PDE and a cyclic AMP PDE. Cyclic GMP-stimulated PDE had Km values of 367 microM for cyclic AMP and 24 microM for cyclic GMP, and low concentrations (1 microM) of cyclic GMP increased the affinity of the enzyme for cyclic AMP (Km = 13 microM) without changing the Vmax...

A homogeneous preparation of cyclic CMP phosphodiesterase (Helfman, D. M., Shoji, M., and Kuo, J. F. (1981) J. Biol. Chem. 256, 6327-6334) was found to catalyze the hydrolysis of both pyrimidine and purine cyclic 2':3'- and 3':5'-nucleotides. Hydrolysis of cyclic 2':3'-nucleotides resulted in the formation of both 2'- and 3'-nucleotides, although relative amounts of the products were variable. ...

INTRODUCTION ................ ROLE OF CYCLIC AMP IN REGULATION OF GENE EXPRESSION .......... A Positive Element in Gene Expression ...................................... CRP .......................... :........................................... Direct stimulation of gene expression ...................................... Possible Role As a Negative Element .........................................

clinical and epidemiological studies have shown that a diet rich in fruits and vegetables is associated with a decreased risk of cardiovascular diseases, cancers and other related disorders. these beneficial health effects have been attributed in part to the presence of antioxidants in dietary plants. therefore screening for antioxidant properties of plant extracts has been one of the interests...

a number of redox-active coordination polymers (cps) or metal-organic frameworks (mofs) have been successfully synthesized using transition metals and bridging ligands. this article aims to deal with gathering the aforementioned disperse issues regarding the electroactive cps. it also goes towards illustrating the effects of various factors on the electrochemical behavior of cps including natur...

A protein kinase activated by cyclic nucleotides was purified from beef heart. Upon exposure to adenosine 3':5'-cyclic monophosphate (cyclic AMP) during gel filtration on Sephadex G-200, the protein kinase dissociated into a cyclic nucleotide-independent protein kinase and a cyclic nucleotide-binding protein. A similar or identical cyclic nucleotide-independent protein kinase could be obtained ...