abstract background: the intracytoplasmic sperm injection procedure ending with total fertilization failure is very distressfull event for both the clinician and the patient. objective: the aim of this study was to identify independent factors which could be used to identify total fertilization failure before the day of intracytoplasmic sperm injection. materials and methods: this was a retrosp...

Intracytoplasmic Sperm Injection (ICSI) is an assisted reproductive technique [4] (ART) initially developed by Dr. Gianpiero D. Palermo [5] in 1993 to treat male infertility [6]. It is most commonly used in conjunction with in vitro [7] fertilization [8] (IVF) or a less commonly used technique called zygote intrafallopian transfer [9] (ZIFT). In natural fertilization [8], the sperm [10] must pe...

BACKGROUND Testis tissue xenografting and the resultant sperm in a xenograft may provide a unique approach to rescue the genetic material of males that die prematurely and is a model for the study of human spermatogenesis and can represent an alternative approach for fertility preservation in cancer patients. This study was aimed to evaluate the xenogenic dog sperm in formation of male pronucle...

Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of chromatin in condensed sperm, we developed a novel combined heparin-reduced gl...

objective: globozoospermia is a rare type of teratozoospermia with incidence of 0.1% among infertile individuals. phospholipase c zeta (plcζ) and postacrosomal sheath ww domain binding protein (pawp) are the main candidates in sperm taking responsibility for oocyte activation during fertilization. therefore, we aimed to evaluate the expression of these two genes at rna and protein levels in glo...

Abstract Study question How should men with globozoospermia be managed in Assisted Reproductive Technologies (ART)? Summary answer IMSI (intracytoplasmic morphologically selected sperm injection), without oocyte activators (OA) allows to obtain a live birth, even when there is mutation of DPY19L2 gene. What known already Globozoospermia rare syndrome found less than 0.1% the infertile populatio...

Background: The cause of fertilization failure in IVF is most often due to the absence of sperm penetration but the reason why fertilization does not occur when intracytoplasmic sperm injection (ICSI) is applied is not clear. However some intrinsic oocyte problems may also be responsible for fertilization failure. It has been reported that 13% of unfertilized oocytes after IVF show morphologica...