introduction: biocompatibility is a desirable feature for root-end filling materials. in this study we aimed to compare a new material called cold ceramic (cc) with intermediate restorative material (irm) and mineral trioxide aggregate (mta) using methyl-tetrazolium bromide (mtt) assay. materials and methods: the materials were tested in fresh and set states: (n=108). the cytotoxicity was compa...

The current study sought to assess the antitumor, anticancer, and antioxidant efficacy of Ulva lactuca-mediated selenium nanoparticles by using an in vitro model human hepatocellular carcinoma (HepG-2 cells) HAV HM175 strain hepatitis A virus, with evaluation activity conducted DPPH assay. showed promising cytotoxicity at highest concentrations 250 500 µg/mL, viability rates 19.43 8.75% for can...

ABSTRACT: Actinomycetes are one of the important secondary metabolite producers. Researchers focused on exclusive marine areas for isolation and identification actinomycetes. The present study Nocardiopsis dassonovillei (ON627850) from TS Pettai region. potential strainTSP1 showed effective antibacterial activity against Haemophilus influenza. TSP1 isolates IC50 value 75.22 μg/ml antioxidant de...

Analyzing the effects on cell growth inhibition and/or cell death has been an important component of biological research. The MTS assay and LDH-based cytotoxicity assays are two of the most commonly used methods for this purpose. However, data here showed that MTS cell proliferation assay could not distinguish the effects of cell death or cell growth inhibition. In addition, the original LDH-ba...

Background: In addition to chromium release assay, flow cytometric methods have been described to assess in vitro cell-mediated cytotoxicity. In this report, we describe a new flow cytometric approach for determination of in vitro cell-mediated cytotoxicity utilizing three-color flow cytometric assay. Methods: This method is based on monoclonal antibody staining of either effector or target cel...

Background and Aim: Resin cements are used widely in restorative dentistry regardless of their biocompatibility. The aim of this study was to compare the cytotoxicity of two categories of dental cements consisting of three chemically set cements (Fuji I, Fuji PLUS and Harvard) and two dual curing cements (BisCem and Duo-Link) by use of MTT assay. Materials and Methods: In this experimental s...

AIM To evaluate a new enzyme immunoassay (EIA) method for detection of Clostridium difficile toxin by comparing it to cytotoxicity assay. To investigate the nature of false negative and false positive EIA results by evaluating clinical and therapeutic parameters. METHODS 737 consecutive diarrhoeal specimens collected from patients clinically suspected of having C difficile colitis were tested...

The present work was designed to investigate the effect of palladium nanoparticles (PdNPs) on human skin malignant melanoma (A375) cells, for example, induction of apoptosis, cytotoxicity, and DNA damage. Diseases resulting from dermal exposure may have a significant impact on human health. There is a little study that has been reported on the toxic potential of PdNPs on A375. Cytotoxic potenti...

Treatment for cancer is often challenging and various interventions may have detrimental effects. Due to this, the development of less harmful alternatives such as herbal medicine essential. The present study aims determine leaf phytoconstituents bioactivities Mallotus cumingii Müll.Arg against cells through utilization MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assa...