1. Vitamin D deficiency was induced in anicteric Wistar rats, Wistar rats before common bile-duct ligation was performed and Gunn rats with unconjugated hyperbilirubinaemia. 2. The effect of ultraviolet radiation on plasma concentrations of 25-hydroxy-vitamin D was studied in a group of control anicteric Wistar rats (n = 11), Wistar rats 8 days after common bile-duct ligation (n = 7) with conju...

V(D)J recombination is responsible for the de novo creation of antigen receptor genes in T- and B-cell precursors. To the extent that lymphopoiesis takes place throughout an animal's lifetime, recombination errors present an ongoing problem. One type of aberrant rearrangement ensues when DNA sequences resembling a V(D)J joining signal are targeted by mistake. This study investigates the type of...

T HE D ANTIGEN has been detected on the surface of Rh( D) leukocytest by a variety of indirect technics. Dausett, Colombani and Evelin1 used the methods of antiglobulin consumption and anti-D absorption by leukocytes. Jankovi#{233} and Lincoln showed fluorescence of Rh(D) leukocytes with fluorescein conjugated antihuman globulin after the exposure of leukocytes to anti-D serum. Hollman was able...

Long-term-cultured poly(Tyr, Glu)-poly-D,L,-Ala-poly-Lys [(T,G)-A--L]-reactive T cells and clones derived from (high responder x low responder)F1 [(C57BL/6 x A/J)F1] mice were shown to recognize (T,G)-A--L presented by cells from low responder strain A/J mice. The antigen-presenting determinant(s) that allowed recognition of (T,G)-A--L by such T cell clones was controlled by the I-A subregion o...

Agents known to interact with either microtubules or microfilaments influenced the antigen-induced release of histamine from the leukocytes of allergic individuals. Deuterium oxide (D(2)O) which stabilizes microtubules and thereby favors their formation enhanced histamine release markedly. Concentrations as low as 5% increased antigen-induced release somewhat while concentrations as high as 75%...

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Abstract Mucosal-Associated Invariant T (MAIT) and Natural Killer (NKT) cells demonstrate that some cell transcriptional fates are driven by the antigen receptor (TCR). Though activation through TCR is integral to differentiation, contribution of sequence features other remains yet be defined. In this study, we identify how αβ V(D)J recombination affects differentiation at single-cell resolutio...

Abstract Respiratory Syncytial Virus (RSV) is a leading cause of morbidity and mortality in children, due part to their distinct immune system, characterized by impaired induction T-helper 1 (Th1)□immunity. Here we describe cationic adjuvant formulation (CAF)-08, liposomal vaccine tailored induce Th1 immunity early life via synergistic engagement Toll-like Receptor 7/8 the C-type lectin recepto...

BACKGROUND The aim of the evaluation was to investigate the relevance of the HCV-core antigen testing for the diagnosis and monitoring of HCV infections in the daily routine. Up to now, most of the serological diagnostics was performed as determination of antibodies while the determination of activity and the monitoring of antiviral therapy were checked by HCV RNA PCR. METHODS The routine req...

T cells recognize peptides derived from the processing of proteins by antigen presenting cells (APCs) in association with the major histocompatibility complex (MHC) molecules. We have engineered a murine MHC class II antigen presenting molecule consisting of the extracellular domains of I-E(d)alpha and I-E(d)beta chains to which the CD4 T cell immunodominant epitope HA110-120 of the hemagglutin...