background and objectives: biological scaffold resources composed of extracellular matrix (ecm) have been shown to make easy the practical remodeling of various tissues in both animal and human studies. the goal of current study was to make sheet form of ecm from sheep’s urinary bladder. methods: ecm was extracted from sheep’s urinary bladder according to standard method. scanning electron mic...

introduction as a tumor grows, the demand for oxygen and nutrients increases and it grows further if acquires the ability to induce angiogenesis. in this study, we aimed to present a two-dimensional continuous mathematical model for avascular tumor growth, coupled with a discrete model of angiogenesis. materials and methods in the avascular growth model, tumor is considered as a single mass, wh...

Abstract The vasculature is an integral unit of the tissue microenvironment due to providing nutrients and oxygen surrounding cells. Therefore, pro-angiogenic biomaterials have potential improve success a wide range medical therapies, including engineering, wound healing, drug delivery. Herein, we decellularized bovine spinal cord meninges with Triton X-100 digested them pepsin obtain hydrogel ...

Kidney transplantation is the only potentially curative treatment for patient facing end-stage renal disease, and it is now routinely used. Its use is mainly limited by the supply of transplantable donor organs, which far exceeds the demand. Regenerative medicine and tissue engineering offer promising means for overcoming this shortage. In the present study, we developed and validated a protoco...

BACKGROUND Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. α-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with α-galactosidase. MATERIALS AND METHODS...

End stage renal disease (ESRD) is a common disease, which relates to nearly 600 million people in the total population. What is more, it seems to be a crucial problem from the epidemiological point of view. These facts lead to a further necessity of renal replacement therapy development connected with rising expenditures for the health care system. The aim of kidney tissue engineering is to dev...

PURPOSE To design a whole-kidney a cellular matrix scaffold using peristaltic pump perfusion and to ascertain the retention of extra cellular proteins by the scaffold. MATERIALS AND METHODS Male Sprague-Dawley (SD) rats weighing 200-250 g were used. Intravenous catheters were inserted into the renal artery followed by perfusion of decellularization solution using a peristaltic pump. After dec...

We developed a nucleus pulposus cell (NPC)-modulated decellularized small intestinal submucosa (SIS) scaffold, and assessed the ability of this material to prevent Intervertebral disc degeneration (IVD) degeneration. Decellularized porcine SIS was squashed into particles and the biological safety and efficiency of these particles were evaluated. Next, SIS particles were seeded with rabbit NPCs,...

background: recurrence rate of rotator cuff tears is still high despite the improvements of surgical techniques, materials used and a better knowledge of the healing process of the rotator cuff tendons. large to massive rotator cuff tears are particularly associated with a high failure rate, especially in elderly. augmentation of rotator cuff repairs with extracellular matrix or synthetic patch...

RATIONALE More than 25 million individuals have heart failure worldwide, with ≈4000 patients currently awaiting heart transplantation in the United States. Donor organ shortage and allograft rejection remain major limitations with only ≈2500 hearts transplanted each year. As a theoretical alternative to allotransplantation, patient-derived bioartificial myocardium could provide functional suppo...