The objective of the study was to examine the effect of fetal calf serum (FCS) and mid estrus cow serum (ECS) in culture media on in vitro maturation (IVM) and in vitro fertilization (IVF) of oocytes from crossbred cattle in the tropics. Oocytes from abattoir ovaries were cultured in three different maturation media at 39C temperature, 5% CO2 tension with maximum humidity for 24 h. TCM-199 cont...

In the in vitro production (IVP) of mammalian embryos, the efficiency of fertilization and post-fertilization development is influenced markedly by in vitro maturation (IVM) conditions (Fukui and Ono, 1989). Mammalian oocytes can spontaneously undergo germinal vesicle breakdown and subsequent formation of metaphase-II stage during maturation and almost similar development occurs in both in vivo...

Background and purpose: Media literacy is defined as the ability to access, analyze, and evaluate the messages transferred by the media, which has the various dimensions, including the creativity, critical thinking and problem solving. This study was conducted with the aim of investigating the media literacy in students of Mazandaran University of Medical Sciences. Material and Method: In this...

Vitamin A (all-trans retinol) is an important antioxidant whose role in embryo development in vitro and in vivo is well established. Oxidative stress is a major cause of defective embryo development. This study evaluated the effects of all-trans retinol supplementation to maturation and embryo culture media under different gaseous environments on the development of ovine oocytes and embryos in ...

The objective of the present study was to evaluate the effect of hyaluronan (HA) during IVM on meiotic maturation, embryonic development, and the quality of oocytes, granulosa cells (GC), and obtained blastocysts. COCs were matured in vitro in control medium and medium with additional 0.035% or 0.07% of exogenous HA. The meiotic maturity did not differ between the analysed groups. The best rate...