Mass spectrometry of intact membrane protein complexes requires removal of the detergent micelle by collisional activation. We demonstrate that the necessary energy can be obtained by adjusting the degree of collisional cooling in the ion source. This enables us to extend the energy regime for dissociation of membrane protein complexes.

Abstract Ethanol has a vast consumption around the world. Many researches confirmed some adverse effect of this component on human health. In addition, recent studies showed significant alteration in both cellular population, and protein profile of human foreskin fibroblast cell line (HFFF2) in the specific dosage of ethanol. Here, the role and interaction of some proteins (characterized by sig...

Fragmentation of molecules under collision-induced dissociation (CID) conditions is not well-understood. This may make interpretation of MSMS spectra difficult and limit the effectiveness of software tools intended to aid mass spectral interpretation. Density Functional Theory (DFT) has been successfully applied to explain the thermodynamics of fragmentation in the gas phase by the modelling th...

BACKGROUND We conducted a 1-year follow-up of an original mail survey of early reactions to the World Trade Center disaster. METHOD Of the 75 subjects originally surveyed, 58 (77%) responded. The survey included measures of dissociation (Dissociative Experiences Scale, Cambridge Depersonalization Scale, Clinician-Administered Dissociative States Scale), post-traumatic stress (Impact of Event ...

Judith Herman (1992) famously observed, “The knowledge of horrible events periodically intrudes into public awareness but is rarely retained for long. Denial, repression, and dissociation operate on a social as well as an individual level” (p. 2). The truth of Herman’s observation makes editing the Journal of Trauma & Dissociation (JTD) all that more sweet–for in creating this journal we are ta...

the present study is to study linear free energy relationships (lfers) for dechlorination ratesin cement/fe(ii) slurries of eight chlorinated hydrocarbons including carbon tetrachloride, chloroform produced from carbon tetrachloride, 1,1,1-trichloroethane, trichloroethylene produced from 1,1,2,2-tetrachloroethane, tetrachloroethylene, trichloroethylene, 1,1-dichloroethylene, and vinyl chloride,...

Electron paramagnetic resonance (EPR) spectroscopy was used to probe the conformational dynamics of the N-terminal arms of the umuD gene products. We determined that the arms of UmuD(2) display a large degree of motion, are largely unbound from the globular C-terminal domain, and that the free energy of dissociation is +2.1 kJ mol(-1).

this research was performed to study the effect of murray red gum tree age (4, 6, 8 years) on the chemical components, viscosity and the cellulose degree of polymerization. the eucalyptus trees were cut at the ages of 4, 6, and 8 from hand planted forests. the contents of cellulose, hemicellulose, lignin, extractives and ash for each age were then determined. the results show that with increasi...

In this research complexation of dioxovanadium(V) with nitrilotriacetic acid (NTA) at differentionic strengths (0.1 to 1.0 mol dm-3) of sodium perchlorate as supporting electrolyte and pH rangeof 1.00-2.50 at 25 °C has been investigated by a combination of potentiometric and UVspectrophotometric measurements. Stability constants of two species, V0211L- and VO2H2L havebeen calculated. Ionic stre...