In this study, the interaction of the anthracycline antibiotic doxorubicin with DNA-HMG 1 complex was investigated employing UV/VIS spectroscopy, thermal denaturation and DNA cellulose chromatography techniques. The results indicated that the binding of doxorubicin to the protein reduces its Tm in a dose dependent manner. Although doxorubicin protects free DNA against thermal denaturation ...

The purpose of the study was to screen phytochemicals and assess antioxidant as well anti-inflammatory properties Vitex pinnata leaf extracts. Alkaloids, glycosides, tannins, terpenoids, saponins, phlobatannins, steroids, quinine, phenol, coumarins, flavonoids were found during phytochemical investigation. V. extract exhibited an IC50 value 154.33 µg/ml in DPPH free radical scavenging assay (st...

For minor and major DNA alleles, coamplification at lower denaturation temperature-PCR (COLDPCR) selectively enriches minority alleles from mixtures of wild-type and mutation-containing sequences without a priori knowledge of the type or position of a mutation within a sequence (1 ). Since its inception (2 ), COLD-PCR has been employed in a variety of applications, including cancer, prenatal di...

Of the different kinds of denaturation of proteins, that by heat has been investigated most thoroughly. The theory has been put forward that the loss of the zwitter ion structure by an interaation of the ionogenic groups is the most important of the electrochemical changes which a protein undergoes by the action of heat (Pauli (6), Cohn (2)). The question as to whether other structural changes ...

We use a master equation approach based on the Poland-Scheraga free energy for DNA denaturation to investigate the (un)zipping dynamics of a denaturation wedge in a stretch of DNA, that is clamped at one end. In particular, we quantify the blinking dynamics of a fluorophore-quencher pair mounted within the denaturation wedge. We also study the behavioural changes in the presence of proteins, th...

Possible differences between two representative non two-state thermal unfolding mechanisms of protein are discussed concerning differential scanning calorimetry. Numerical simulations showed that, by DSC measurement, it is hard to discriminate between the independent model, which assumes independent unfolding domains in a protein, and the sequential model, which assumes intermediate(s) between ...

The mechanism by which RapiGest SF improves the speed and completeness of digestion is illustrated in Figure 1. Mild protein denaturation opens protein structure and exposes the proteolytic sites to enzymatic cleavage. Enzymes are more resistant to denaturation than common proteins and remain active in RapiGest SF solutions. More complete denaturation of globular proteins can be accomplished by...

Heats of thermal denaturation of chromatin core particles and core particles with covalently crosslinked histones were measured by differential scanning calorimetry. The additional stabilization of the nucleoprotein complex by crosslinking is not reflected in the transition enthalpy. The contribution of protein denaturation to the total heat was estimated by comparison of core particles with co...

The stability of calf skin collagen (CSC) type I during thermal and chemical denaturation in the presence of glycerol was investigated. Thermal denaturation of type I collagen was performed in the presence of glycerol or in combination with urea and sodium chloride. The denaturation curves obtained in the presence of urea or sodium chloride retained their original shape without glycerol. These ...

We present the software CDpal that is used to analyze thermal and chemical denaturation data to obtain information on protein stability. The software uses standard assumptions and equations applied to two-state and various types of three-state denaturation models in order to determine thermodynamic parameters. It can analyze denaturation monitored by both circular dichroism and fluorescence spe...