objectives: this study aimed to compare the in vitro cytotoxic activity of propolis, a bioactive material made by the honeybee, and calcium hydroxide (ch) and their effect on formation of mineralized nodules by human dental pulp stem cells (hdpscs). methods: in this in vitro study, hdpscs were obtained from the cellular and molecular oral biology laboratory of school of dentistry, shahid behesh...

In recent years, mesenchymal cell-based products have been developed to improve surgical therapies aimed at repairing human tissues. In this context, the tooth has recently emerged as a valuable source of stem/progenitor cells for regenerating orofacial tissues, with easy access to pulp tissue and high differentiation potential of dental pulp mesenchymal cells. International guidelines now reco...

AIMS Our aims were to isolate dental pulp stem cells, to cultivate them in various media and to investigate their basic biological properties and phenotype. METHODS 16 lines of dental pulp stem cells (DPSCs) were isolated from an impacted third molar. After enzymatic dissociation of dental pulp, DPSCs were cultivated in modified cultivation media for mesenchymal adult progenitor cells contain...

In this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and re-transplanted into immunocompromised mice to generate a dentin-...

During the last decade, stem cells emerged as a powerful instrument for regenerative medicine. Stem cells have the essential ability to renewal and differentiate into other cell lineages. Besides the polemic about the use of embryonic stem cells, adult stem cells represent a valid alternative avoiding ethical concerns. Different from others adult stem cells, which involved invasive procedures t...

BACKGROUND Successful pulp regeneration depends on identification of pulp stem cells capable of differentiation under odontoblastic lineage and producing pulp-dentinal like structure. Recent studies demonstrate that platelet-derived growth factor (PDGF) plays an important role in damage repair and tissue regeneration. The aim of this study was to identify a subpopulation of dental pulp cells re...

Stem cell biology has become an important field in regenerative medicine and tissue engineering therapy since the discovery and characterization of mesenchymal stem cells. Stem cell populations have also been isolated from human dental tissues, including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, dental follicle progenitor cells, an...

Background Currently, the use of stem cells from permanent (DPSCs) and deciduous teeth (SHEDs) has enabled many therapeutic advances. Despite numerous sources of stem cells, DPSCs and SHEDs have shown several advantages, since the isolation method is noninvasive and cells present rapid expansion in vitro [1]. DPSCs have shown the capacity to form a dentin-pulp like complex. Due to its high oste...

BACKGROUND: Pulp damage can lead to dentinogenesis impairment, irreversible pulpitis, or pulp necrosis. Despite being the most used endodontic procedure treat damaged pulp, root canal therapy only results in nonvital teeth which are prone fractures and secondary infection. Pulp-dentin regeneration has a potential regenerate structure similar normal pulp-dentin complex, be achieved by combining ...