The cyclic AMP-dependent protein kinase (isoenzymes I and II) was measured using a synthetic peptide analog of the porcine hepatic phosphorylation site sequence (Leu-Arg-Arg-Ala-Ser-&eu-Gly). Epinephrine caused a timeand dose-dependent activation of the hepatic cyclic AMP-dependent protein kinase and inactivation of pyruvate kinase. Maximal activation of the protein kinase occurred within 1 min...

The cyclic AMP-dependent protein kinase (isoenzymes I and II) was measured using a synthetic peptide analog of the porcine hepatic phosphorylation site sequence (Leu-Arg-Arg-Ala-Ser-&eu-Gly). Epinephrine caused a timeand dose-dependent activation of the hepatic cyclic AMP-dependent protein kinase and inactivation of pyruvate kinase. Maximal activation of the protein kinase occurred within 1 min...

Insulin-like growth factor (IGF)-I and -II have been cloned from a number of teleost species, but their cellular actions in fish are poorly defined. In this study, we show that both IGF-I and -II stimulated zebrafish embryonic cell proliferation and DNA synthesis in a concentration-dependent manner, whereas insulin had little mitogenic activity. Affinity cross-linking and immunoblotting studies...

We have investigated the activation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) in cultured bovine adrenal medullary cells. The activation was assayed as an increase in the Ca(2+)-independent (autonomous) activity of CaM kinase II, using the synthetic substrate Syntide-2. Incubation of cells with acetylcholine increased the Ca(2+)-independent activity in a time (20 sec to 5.0...

A Ca2+/calmodulin (CaM)-dependent multifunctional protein kinase has been isolated from Aspergillus nidulans and purified to homogeneity. Unlike any CaM-dependent multifunctional protein kinase described previously, the native enzyme from Aspergillus behaves as a monomer. The calculated molecular weight is 41,200. NaDodSO4/PAGE reveals a single protein band with an apparent Mr of 51,000. Two-di...