We demonstrate a combination of single molecule force spectroscopy and solvent substitution that captures the presence of solvent molecules in the transition state structure. We measure the effect of solvent substitution on the rate of unfolding of the I27 titin module, placed under a constant stretching force. From the force dependency of the unfolding rate, we determine Deltax(u), the distanc...

We demonstrate a combination of single molecule force spectroscopy and solvent substitution that captures the presence of solvent molecules in the transition state structure. We measure the effect of solvent substitution on the rate of unfolding of the I27 titin module, placed under a constant stretching force. From the force dependency of the unfolding rate, we determine xu, the distance to th...

[18f]kf, considered as a reference standard for positron emission tomography of the skeletal system, was produced by the bombardment of enriched water ([18o]h2o>95%) with high energy protons in nrcam cyclotron. after the extraction of fluoride from enriched water as kf, and passing it through microbial filter and fine adjustment of acidity and osmolality, it was converted into an injectable for...

The Schoeller model for calculating rate of CO2 production (rCO2) from doubly labeled water (DLW) relies on the assumption that deuterium and oxygen-18 overestimate body water by 4 and 1%, respectively. However, the deuterium-to-oxygen-18 dilution space ratio (DSR) varies considerably, and it is unknown whether this is due to analytical or biological sources. From 161 published values in adult ...

The production of deuterated methane by Methanobacterium thermoautotrophicum in H2O-D2O mixtures was examined by high-resolution mass spectrometry. The hydrogen in the methane arose solely from water and not from hydrogen gas. Hydrogen gas served only as an electron source in methanogenesis. A whole-cell product isotope discrimination of 1.5 favoring hydrogen over deuterium was observed in meth...

The spatial arrangement of polymers in Sitka spruce (Picea sitchensis) was investigated by NMR proton spindiffusion studies, supplemented by deuterium-exchange experiments monitored by FTIR spectroscopy. The FTIR spectra of earlywood sections after vapour-phase exchange with deuterium oxide showed that 43% of the hydroxyl groups were accessible to deuteration. This value is lower than predicted...

Investigation of the metabolism and fate of steroid hormones has been greatly facilitated by isotope stably incorporated into the substance t,o be studied. For many purposes the isotopes of hydrogen are decidedly advantageous in that the chemical synthesis of the labeled steroid can be easily and quickly achieved with a minimum of manipulation. Moreover, since the endogenous pool of the hormone...

Cucurbit[7]uril (CB[7]) encapsulates adamantyl and trimethylsilyl substituents of positively charged guests in dimethyl sulfoxide (DMSO). Unlike water or deuterium oxide, addition a selection alkali alkali-earth cations...

BACKGROUND In developing countries where access to water and food is not guaranteed, women may have to travel long distances or engage in intense physical activities to gather food. This may compromise their water requirements and overall nutritional status. The aim of the study was to determine water turnover, physical activity and body composition among women in Kenya and to describe the diff...

The 2H/1H and 18O/16O isotope ratios in rainwater bring a lot of information about the fractionation water molecules hydrosphere. relationship between an area characterised by local meteoric line, which is known as reliable reference value for studies related to identification recharge source groundwater climate change investigations. This study aims establish line Long Khanh city (LK LMWL) per...