Although the biochemical changes that occur during cell differentiation are well-known, less known is that there are significant, cell-wide physical changes that also occur. Understanding and quantifying these changes can help to better understand the process of differentiation as well as ways to monitor it. Digital holographic microscopy (DHM) is a marker-free quantitative phase microscopy tec...

Scattering and absorption belong to the major problems in imaging the internal layers of a biological specimen. Due to the structural inhomogeneity of the specimen, the distribution of the structures in the upper layers of a given internal structure of interest is different from the lower layers that may result in different interception of scattered light, falling into the angular aperture of t...

Seasonal forecasts of sea surface temperature (SST) have become increasingly important tools in recent years for reef managers to help inform and coordinate management responses mass coral bleaching events. This manuscript presents new operational thermal stress forecast products prediction risk, based on the seasonal ensemble system ACCESS-S1 (Australian Community Climate Earth System Simulato...

Human milk banks (HMB) are responsible for screening and recruiting donors with surplus to their own infant’s needs, followed by transporting, heat-treating (pasteurising) microbiologically confirming the donor human (DHM) is safe issue vulnerable infants. Maintaining safety quality of DHM vital requirements in HMB operations. must be maintained ideal temperature conditions throughout whole per...

We have developed a drug delivery nanosystem based on chitosan and capsaicin. Both substances have a wide range of biological activities. We investigated the nanosystem's influence on migration and morphology of Madin Darby canine kidney (MDCK-C7) epithelial cells in comparison to the capsaicin-free nanoformulation, free capsaicin, and control cells. For minimally-invasive quantification of cel...

Myelin figures (MFs) are cylindrical multilamellar lipid tubes that can be found in various healthy and diseased living cells. Their formation and dynamics involve some of the most mysterious configurations that lipid molecules can adopt under certain conditions. They have been studied with different microscopy methods. Due to the frequent coiling of their structure, the usual methods of micros...

Digital holography enables the encoding of complex wavefronts with an intensity measurement, making it possible to recover the quantitative phase for phase-contrast imaging [1] or profilometry [2]. Encoding the wavefront in this fashion leads, however, to the generation of artifacts such as the socalled zero-order, which is the sum of the intensities of the object and reference waves, and the s...

Receptor autoradiography was combined with the retrograde labeling of sympathoadrenal neurons by fast blue to determine whether opiate, serotonin, catecholamine, or cholinergic binding sites could be spatially correlated with preganglionic neurons in the rat intermediolateral cell column (IML) that project to the adrenal gland. [3H]Dihydromorphine (DHM) was used for the visualization of mu opia...

Recent advances in digital technologies, such as high-speed computers and large-format digital imagers, have led to a burgeoning interest in the science and engineering of digital holographic microscopy (DHM). Here we report on a novel off-axis DHM, based on a twin-beam optical design, which avoids the limitations of prior systems, and provides many advantages, including compactness, intrinsic ...