UV irradiation of 1,3-dimethyluracil in ice-matrix yields four dimeric dimethyluracils (2 c) — (5 c) , which could be isolated by preparative thin-layer chromatography. Attemps for structure elucidation of these four dimers by UV, IR, NMR and mass-spectrometric methodes pointed out, that only NMR-spectra give structural evidences; by IR — not by mass-spectra — certain identification of dimers i...

human erythrocyte transketolase (sedoheptulose-7-phosphate: d-glyceraldehyde-3-phosphate, glycolaldehyde transferase, e.c. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 u/mg. sds-page and se-hplc were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. four protein bands corresponding to molecular weights of 32,000, 3...

Pectinases are most significant enzymes of commercial sector. Among all the commercially produced industrial enzymes, pectinases occupy 25% share in overall enzyme market. have wide applications various processes such as fruit juice clarification, wastewater remediation and textile paper industry etc. Pectinase is suitable environment friendly tool nature for sustainable development. Although, ...

This work evaluates the enzymatic production of hydrolases and oxireductases by endophytic fungi isolated from leaves caesarweed (Urena lobata L.), which is a perennial plant that well adapted to Amazon floodplains region, being widely used in hessian sacks other products natural fiber. Fungi strains Fusarium sp. (1290) Corynespora (1291) were reactivated, had their DNA extracted sequenced obta...

The goal is to improve the flavors of Arabica coffee by combining semi-carbonic maceration (SCM) process, with enzymatic process at various lengths and temperature. Five different variations SCM processes were investigated: pulped natural (control); 15 oC for 10 days (SCM-15); 20 (SCM-20); 0.01 g.L-1 pectinases A (polygalacturonase, pectin esterase lyase) 5 (SCM-PA); 0.03 B esterase, lyase ß-gl...

Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and Aspergillus terreus MS105 were screened for plant CWDE such as amylase, pectinase, xylanase and cellulases (β-glucosidase, endoglucanase and filterpaperase) usi...

Jamun fruit comprises of seed and thick pulp. The pectin-protein bond of the thick pulp creates difficulty in making juice. Clear Jamun juice is not available in the market, so there is a need for extraction of juice with maximum yield. The goal of this research is to obtain high yield of clarified juice with the help of Pectinase (Aspergillus aculeatus) enzyme. The study was conducted at diffe...

Several atoxigenic Aspergillus flavus isolates, including some being used as biocontrol agents, and one toxigenic isolate were surveyed for the ability to produce extracellular xylanolytic and pectinolytic hydrolases. All of the tested isolates displayed good production of endoxylanases when grown on a medium utilizing larch xylan as a sole carbon substrate. Four of the tested isolates produced...