A chemically defined medium was developed that could support sporulation and growth of Clostridium perfringens strains ATCC 12916 and H9. This medium consisted of a modification of the basal medium of Boyd et al. plus 0.1% sodium thioglycolate and 0.5% monosodium glutamate. Five other strains grew, but did not sporulate, in this medium. With the addition of more vatamins into the medium, two mo...

Human bronchial epithelium has been maintained in organ culture in serum-supplemented medium for 4 months. After 4 to 6 weeks in culture, various changes in morphology were apparent. There was an increase in autophagic vacuoles in mucous, ciliated, and basal cells, a reduction in the height of the columnar cells, a decrease in the number of goblet mucous cells, and an increase in cells with sma...

Nodal segments from six years old plants of Bauhinia variegata L. were cultured on Murashige and Skoogs (MS) medium supplemented with different concentration and combinations of BAP and IBA. Explants culture in MS basal medium supplemented with 5mg/1 BAP showed highest rate of shoot multiplication. When in vitro shoots were inoculated on the MS basal medium supplemented with IBA 2mg/l, profusse...

Gastric Leptin is absorbed by duodenal enterocytes and released on the basolateral side towards the bloodstream. We investigated in vitro some of the mechanisms of this transport. Caco-2/15 cells internalize leptin from the apical medium and release it through transcytosis in the basal medium in a time- temperature-dependent and saturable fashion. Leptin receptors are revealed on the apical bru...

Short, medium, and long latency EMG responses to muscle stretch from triceps surae and anterior tibialis muscles were recorded in normals and in 33 patients with Parkinson's disease. The latencies of all EMG responses except short latency were normal in patients with Parkinson's disease. The integrals of the medium latency responses in the stretched triceps surae muscle were significantly incre...

as follows: 500 ml 230 medium 100 reciprocating movements per min for 5 days at Low purple membrane production is observed under conditions of sufficient aeration: cells were grown in Erlenmeyer flasks containing only 90 ml medium under otherwise in the presence of mM nicotine yielded cells with ml medium) or (90 ml of bacteria-opsin, respectively (‘nicotine [7 ] Basal salt solution contained l...

With the aim to assess regenerative response of six mid late sugarcane clones under in vitro conditions with four different types growth media and research was conducted Sugarcane Hi-tech Laboratory, SRI, DRPCAU, Pusa, Bihar. Shoot apex explant culture these cultivars on M1 (MS basal) M2 (ms basal + 0.5 mgl1 IAA 0.5mgl1 KIN) suggested no definite role medium establishment cultures. selected M3 ...

Study of the requirement of trace elements for the streptomycetes has often led to contradictory results (Woodruff, 1947; Spilsbury, 1948; Johnstone & Waksman, 1948). This is mostly due to the difficulty in preparing a truly basal medium and also because such requirements vary with the medium employed (Thornberry & Anderson, 1948). The streptomycete Streptomyces olivaceus is one of the few micr...

Several characteristics of oviductal cells, cultured under either polarizing or nonpolarizing conditions, were studied. In vitro produced bovine embryos tested the embryotrophic abilities of the respective conditioned media. Conditioned medium from the apical face of polarized cell monolayers supported higher rates of development to blastocyst and expanded blastocysts. In contrast, conditioned ...