DNA manipulation is crucial for many biotechnological prospects and medical applications such as gene therapy. This requires the amplification extraction of from bacteria transfer these molecules into cells, including bacterial mammalian cells. The capacity natural magnesium silicate clay mineral sepiolite to bind makes it a potentially useful tool biotechnological/medical strategies. In additi...

The dynamic nature of micellar nanostructures is employed to form a self-assembled Förster resonance energy transfer (FRET) nanoplatform for enhanced sensing DNA. platform consists lipid oligonucleotide FRET probes incorporated into scaffolds, where single recognition events result in fusion and fission DNA mixed micelles, triggering the fluorescence response multiple rather than pair. In compa...

Loop-mediated isothermal amplification (LAMP) is a gene amplification method which amplifies DNA with high specificity and efficiency under isothermal conditions. Because of its rapidity and simplicity, it is a valuable diagnostic tool in the early detection and identification of infectious diseases. LAMP method is based on the use of a set of four to six specially designed primers spanning six...

We developed a nicking endonuclease dependent DNA amplification (NDA), using Nt.BstNBI to catalyze single-stranded nick on double-stranded DNA, and Bst DNA polymerase to make extension while sealing the nick and displacing the downstream strand. The displaced single-stranded DNA thereby serves as template for primers hybridization and extension, resulting in exponential synthesis of target DNA ...

lysates of the recombinant P. pastoris strains KM71 (pPIC9-MYO)-MutS and GS115 (pPIC9-MYO)-Mut+, which contained the integration of the myostatin gene to chromosomal DNA, was completed. When Taq DNA polymerase was used for amplification, we observed either no or nonspecific amplification products. The positive control sample yielded the 831-bp specific fragment. When the HOTStar Taq DNA polymer...

We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.

AIM The overall aim of this study is to evaluate whole genome amplification of DNA extracted from dried blood spot samples. We wish to explore ways of optimizing the amplification process, while decreasing the amount of input material and inherently the cost. Our primary focus of optimization is on the amount of input material, the amplification reaction volume, the number of replicates and amp...