A filter binding assay was developed that allows measurement of specific binding of trp repressor to operator DNA. The most important feature of this procedure is the concentration and type of salt present in the binding buffer. Using this assay the dissociation constant of the repressor-operator complex was determined to be 2.6 X 10(-9) M, and 1.34 repressor dimers were found to be bound to ea...

The report describes the synthesis, self-association and DNA binding studies of an aromatic tripeptide H-Phe-Phe-Phe-OH (FFF). The peptide backbone adopts β-sheet conformation both in solid and solution. In aqueous solution, FFF self-assembles to form nanostructured aggregates. Interactions of this peptide with calf-thymus DNA (ct-DNA) have been studied using various biophysical techniques incl...

The genome of Escherichia coli is composed of a single molecule of circular DNA with the length of about 47,000 kilobase pairs, which is associated with about 10 major DNA-binding proteins, altogether forming the nucleoid. We expressed and purified 12 species of the DNA-binding protein, i.e. CbpA (curved DNA-binding protein A), CbpB or Rob (curved DNA-binding protein B or right arm of the repli...

The experimental determination of formation constant for interaction of two water soluble phthalocyanines,Cobalt (II) 4,4',4",4'"-tetrasulpho phthalocyanine (CoTsPc ) and Manganese(II) 4,4',4",4"-tetrasulphophthalocyanine (MnTsPc ) , with calf thymus DNA have been studied by UV-Vis spectrophotometric methodat lrnM phosphate buffer , pH 7.4 and at 5 different temperatures 20,25,30,35 and 400CC ....

Transcription of the Bacillus subtilis dra-nupC-pdp operon is repressed by the DeoR repressor protein. The DeoR repressor with an N-terminal His tag was overproduced with a plasmid under control of a phage T5 promoter in Escherichia coli and was purified to near homogeneity by one affinity chromatography step. Gel filtration experimental results showed that native DeoR has a mass of 280 kDa and...

The genome of Escherichia coli is composed of a single molecule of circular DNA with the length of about 47,000 kilobase pairs, which is associated with about 10 major DNA-binding proteins, altogether forming the nucleoid. We expressed and purified 12 species of the DNA-binding protein, i.e. CbpA (curved DNA-binding protein A), CbpB or Rob (curved DNA-binding protein B or right arm of the repli...

In this research, a novel 4,4'-Diaminodiphenyl sulfide-based Schiff base compound [6,6'-((1E,1'E)-((thiobis (4,1-phenylene)) bis(azaneylylidene)) bis(methaneylylidene)) bis(3-(diethylamino) phenol) (A)], which is known to have good biological activity and forms the basis of anticancer drugs, was successfully synthesized. Structural characterization synthesized determined by FT-IR 1H-13C NMR, sp...

A new metal–organic complex (MOC), Mn(L)2·4H2O is successfully designed and synthesized by 2-(1,2,4-Triazol-1-yl)-4-picolinic acid ligand Mn(NO3)2·4H2O in the mixed solvent of methanol water. The was characterized single-crystal X-ray diffraction, element analysis, Powder diffraction(PXRD). binding constant between DNA 9.3 × 104, indicating that fish sperm can bind well. Viscosity experiments p...

Continuous labelling of callus with H-thymidine results in intermittent peaks of H-DNA per chloroplast, showing synchrony of division. The increase in H-DNA could be due to several replication rounds, and the drop to successive plastid divisions without intervening DNA synthesis. The level of DNA-binding proteins in the chloroplast parallels the peaks of plastidal DNA synthesis; such pro...

To overcome early cancer detection challenges, diagnostic tools enabling more sensitive, rapid, and noninvasive are necessary. An attractive target for blood tests is human Ecto-NOX disulfide–thiol exchanger 2 (ENOX2), expressed in most types regularly shed into sera. Here, we developed an electrochemical DNA-based (E-DNA) biosensor that rapidly detects physiologically relevant levels of ENOX2....