The simple helical motif of double-strand DNA (dsDNA) has typically been judged to be uninteresting for assembly in DNA-based nanotechnology applications. In this letter, we demonstrate construction of superstructures consisting of heterogeneous DNA motifs using dsDNA in conjunction with more complex, cross-tile building blocks. Incorporation of dsDNA bridges in stepwise assembly processes can ...

In the last decade, "small" and "integrated" have been keywords in the field of device fabrication as the desire to exploit nanoscale phenomena and make electronic, photonic and magnetic arrays has grown. In an effort to improve resolution and control costs, much work has been dedicated to developing alternatives to conventional microfabrication technology. For this purpose, biomolecular assemb...

A promising application of DNA self-assembly is the fabrication of chromophore-based excitonic devices. DNA brick assembly is a compelling method for creating programmable nanobreadboards on which chromophores may be rapidly and easily repositioned to prototype new excitonic devices, optimize device operation, and induce reversible switching. Using DNA nanobreadboards, we have demonstrated each...

Self-assembly is a common phenomenon in the macro-molecular environment inside the cell and is critical for many cellular functions. Viral capsid assembly has been studied as a key model for self-assembly systems by researchers from different fields. There nonetheless remains a substaintial gap between experimental observations and current models, as the direct measurement of the assembly dynam...

The reliability of methylation measures from the widely used HumanMethylation450 (HM450K) microarray has not been assessed for DNA from dried blood spots (DBS) or peripheral blood mononuclear cells (PBMC), nor for combined data from different studies. Repeated HM450K methylation measures in DNA from DBS and PBMC samples were available from participants in six case-control studies nested within ...

In this paper we examine the computational capabilities inherent in the hybridiza-tion of DNA molecules. First we consider theoretical models, and show that the self-assembly of oligonucleotides into linear duplex DNA can only generate sets of sequences equivalent to regular languages. If branched DNA is used for self-assembly of dendrimer structures, only sets of sequences equivalent to contex...

Methods Infants born to HIV positive mothers were tested according to NACO guidelines. Infants <6 months of age (n=136) were tested by DBS HIV-1 DNA PCR; DBS positive were confirmed by whole blood PCR. Infants 6 to 18 months (n=68) were tested by antibody test and if positive were confirmed by DBS HIV-1 DNA PCR. Detailed history including type of delivery, Single Dose Nevirapine (SDN) and breas...

ABSTRACT Self-assembling DNA complexes have been intensively studied in recent years aiming to achieve bottom-up construction of nanoscale objects. Among them, a DNA complex called DNA tile is known for its high programmability. By using a set of DNA tiles, we are able to self-assemble twodimensional crystals with programmable patterns. This is called algorithmic self-assembly. In order to crea...

The assembly of synthetic, controllable molecules is one of the goals in nanotechnology. The primary objective of this contribution is to selectively immobilize DNA on gold via electric potential control. The self-assembly monolayer (SAM) was prepared with 2-aminoethanethiol (AET) on the gold electrode. A new approach based on electric potential was firstly used to control DNA immobilization co...

This chapter reviews the state-of-the-art in the study of molecular or colloidal systems whose mutual interactions are mediated by DNA molecules. In the last decade, the robust current knowledge of DNA interactions has enabled an impressive growth of self-assembled DNA-based structures that depend crucially on the properties of DNA-DNA interactions. In many cases, structures are built on design...