The prokaryotic CpG-specific DNA methylase from Spiroplasma, SssI methylase, has been extensively used to methylate plasmid DNA in vitro to investigate the effects of methylation in vertebrate systems. Currently available methods to produce CpG-methylated plasmid DNA have certain limitations and cannot generate large quantities of methylated DNA without cost or problems of purity. Here we descr...

neuronal and astroglial cells were prepared from whole brain of three month and 30-month- old rats for study of alterations in the nuclear poly adp-ribosylation and dna breaks with age. the relative purity of the cell preparations was confirmed by the determination of the neurofilament (low molecular weight) and glutamine synthetase content of the cells using elisa. an increase (75%) in the pol...

Despite recent advances in DNA-based genotyping, the microcytotoxicity test is still broadly used for the determination of human leukocyte class I antigens in patients as well as organ donors and also for the detection of HLA antibodies. Excellent purity and viability of peripheral blood mononuclear cells (PBMC) are essential for reliable HLA typing results. Background staining and cell loss ca...

Metagenomic DNA from sediments of selective estuaries of Goa, India was extracted using a simple, fast, efficient and environment friendly method. The recovery of pure metagenomic DNA from our method was significantly high as compared to other well-known methods since the concentration of recovered metagenomic DNA ranged from 1185.1 to 4579.7 µg/g of sediment. The purity of metagenomic DNA was ...

Sodium bisulfite treatment followed by PCR and DNA sequencing is widely considered the gold standard for the analysis of DNA methylation patterns. However, this technique generally requires substantial quantities of genomic DNA as starting material and is often associated with degradation of DNA. Here, we assess the feasibility of performing bisulfite sequencing on DNA isolated from 3-mm diamet...

DNA analyses and DNA-based diagnoses are going forwardly in the commercial deals with shrimp (Penaeus monodon) as well basic research. In this regard extraction of is obvious. However, numerous protocols available from animal tissue. Therefore, which technique suitable one for tissue indispensable to justify. current study, a protocol was optimized collected Batiaghata, Khulna. Muscle Tissue (M...

Neuronal and astroglial cells were prepared from whole brain of three month and 30-month- old rats for study of alterations in the nuclear poly ADP-ribosylation and DNA breaks with age. The relative purity of the cell preparations was confirmed by the determination of the neurofilament (low molecular weight) and glutamine synthetase content of the cells using ELISA. An increase (75%) in th...

DNA for use in polymerase chain reactions (PCR) and other molecular testing is most commonly isolated from anticoagulated blood. With the need to minimize blood volume used in laboratory testing, the sharing of samples among laboratories for a variety of different tests and extraction of DNA from blood clots that would otherwise be waste products is becoming increasingly important. Several meth...

Many biochemical, biomedical, and material applications hinge on the ability to effectively separate purify nanoscale materials. Though this need is largely addressed with biological macromolecules using a variety of chromatographic electrophoretic purification techniques, such techniques are usually laborious, time-consuming, often require complex costly instalments that inaccessible most labo...