Introduction: Occurrence of detectable amounts of viral antigen or viral particles in the blood of infected patients with Hepatitis B virus (HBV) is a significant characteristic of HBV infection. Detection of HBV antigen or its DNA among individuals of a community is a crucial factor to know the burden of HBV infection. Hepatitis B surface antigen (HBsAg) is a suitable marker of HBV infection b...

The development of new techniques for the detection microRNAs (miRNAs) is highly desirable. Herein, a crystalline three-component covalent organic framework (COF) termed EB-TAPB-TFP COF was synthesized under solvothermal conditions utilizing 1,3,5-triformylphloroglucinol, 1,3,5-tris(4-aminophenyl)benzene and ethidium bromide as monomers. Interestingly, can be self-exfoliated into two-dimensiona...

Sago palm (Metroxylon sago Rottb.) is one of the carbohydrate-producing crops that has great potential in supporting food security programs. This study aims to determine morphological and genetic diversity based on mat-K gene marker 15 accessions pal have been collected by Research Consortium. research was conducted preparing plant samples, extracting DNA, amplifying DNA with a PCR tools, visua...

Peptide nucleic acid (PNA) oligomers can be employed as site-specifi c openers of the DNA double helix to locally expose a designated marker sequence inside duplex DNA. The opened DNA site is then hybridized to a circularizable oligonucleotide probe, which is subsequently closed by DNA ligase. This way, the marker sequence from the DNA duplex of interest can be isothermally amplifi ed by a vari...

the purpose of this study was to identify the rate of polymorphism in bm6444, inra13 and orahh35 microsatellite markers associated with inhibin gene in sanjabi sheep. for this purpose, blood samples were taken randomly from 100 sanjabi sheep (78 females and 22 males) at mehregan breeding station at kermanshah. modified salting out method was employed for extracting dna. subsequently, polymerase...

We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping scheme is to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA. Each of these probes will define a locus. Loci can be expanded or contracted...

The biological fate of temperate phage HP1 deoxyribonucleic acid (DNA) was followed after uptake by defectively lysogenic competent Haemophilus influenzae cultures. The similar inactivation kinetics of three single phage genetic markers and of their triple combination indicated a complete rather than partial destruction of about half of the adsorbed DNA molecules. Intracellular DNA breakdown pr...

Sex identification is a problem in research and conservation. It can often be solved using a DNA test but this is only an option if a sex-specific marker is available. Such markers can be identified using the amplified fragment length polymorphism (AFLP) technique. This is usually a taxonomic method, as it produces a DNA fingerprint of 50-100 PCR bands. However, if male and female AFLP products...

We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping scheme is to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA. Each of these probes will define a locus. Loci can be expanded or contracted...

F OLLOWING absorption of DNA by competent cells, genetic transformation ensues with a probability that is characteristic for each particular genetic marker (HOTCHKISS and MURMUR 1954; LERMAN and TOLMACH 1957). Thus, specific incorporation, defined as the amount of DNA which must be incorporated in order to obtain one transformant, varies according to the genetic marker employed. With the develo...