in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

Various approaches to the self-assembly of molecules have been introduced already 2), 3), 4), 5), 6), . A step further toward flexible design and construction of precisely defined molecules are approaches to programmable self-assembly . In order to allow arbitrary programming, a sufficient solution of the negative design problem 5), 9) is needed. We present a computer program which translates f...

This chapter introduces various visualization (i.e., graphical representation) schemes of symbolic DNA sequences, which are basically represented by character strings in conventional sequence databases. Several visualization schemes are reviewed and their characterizations are summarized for comparison. Moreover, further potential applications based on the visualized sequences are discussed. By...

A colorimetric assay of sequence-specific DNA cleavage by bleomycin (BLM) derivatives is a promising methodology for facilitating the development DNA-associated drugs with antitumor activity. Double-stranded DNA-functionalized gold nanoparticles (dsDNA-AuNPs) possessing BLM-mediated sites were designed. The front cover highlights non-fluorescence-based analytical method that allows visual scree...