THE prime indication for transplanting a graft of the patient's own corneal tissue from one side to the other is the presence of a dense extensive corneal scar on a potentially seeing eye, while the other side has a clear cornea in an eye with an intractable and blinding lesion in the intra-ocular media or fundus. Such cases are not commonly seen, but have been reported mainly because the best ...

THE use of corneal grafting has increased considerably in the last 15 years and it was therefore thought of interest to survey the frequency of infection and the bacterial strains found post mortem in the conjunctival sac and on the cornea in eyes suitable for use as donor eyes. We have also investigated the possibility of reducing the frequency of infection by pre-treatment with antibiotics. T...

PURPOSE To report a case of corneal graft failure due to epithelial ingrowth after an uneventful combined Descemet stripping automated endothelial keratoplasty (DSAEK) and phacoemulsification cataract surgery with intraocular lens implant treated successfully with a repeat DSAEK. METHODS A 77-year-old male patient underwent combined DSAEK and phacoemulsification with intraocular lens implant ...

Purpose Fungal infections post keratoplasty due to contamination of the donor corneal graft have become important issues that need to be addressed. Here we report a case of fungal keratitis and endophthalmitis post penetrating keratoplasty (PKP) due to fungal contamination of the donor corneal graft. Observations We present a 52-year-old male who underwent PKP with a donor corneal graft that ...

PURPOSE To identify differentially expressed genes in keratoconus (KC) corneal fibroblasts. METHODS Stromal keratocytes (having a fibroblast morphology) from KC keratoplasty specimens and eye bank donor corneas were isolated and expanded using a serum containing medium. RNA was isolated from three KC fibroblast cultures and five eye bank donor cornea fibroblast cultures. The targets from the ...

We describe herein a case of an impending corneal perforation with a large descemetocele in a patient with previous penetrating keratoplasty (PKP) that subsequently was treated with an emergent lamellar keratoplasty using frozen preserved cornea. A 76-year-old male patient, who had a PKP, presented with a completely whitish and edematous graft accompanied by large epithelial defects. Although a...

PURPOSE To analyze the quality of the corneas evaluated by slit lamp examination in the Eye Bank of the Hospital de Clínicas UFPR and its relation to donor age and cause of death. METHODS Analysis of 492 corneas, evaluated in BTO HC-UFPR between August 2006 to August 2008. Each cornea was classified regarding the quality as: very good, good, regular and bad; and according to clarity, epitheli...

PURPOSE To determine whether complement-derived SC5b-9, the soluble nonlytic-fluid phase of the membrane attack complex, can be generated in normal human corneas when they are injured with lipopolysaccharide (LPS), ribitol teichoic acid (RTA) immune complexes, acid, or alkali. METHODS The experimental cornea of each donor pair was injected with 50 microliters of sterile saline containing 0.5 ...

Corneal endothelium is a single layer of specialized cells that lines the posterior surface of cornea and maintains corneal hydration and corneal transparency essential for vision. Currently, transplantation is the only therapeutic option for diseases affecting the corneal endothelium. Transplantation of corneal endothelium, called endothelial keratoplasty, is widely used for corneal endothelia...