The Escherichia coli SOS response to DNA damage is modulated by the RecA protein, a recombinase that forms an extended filament on single-stranded DNA and hydrolyzes ATP. The RecA K72R (recA2201) mutation eliminates the ATPase activity of RecA protein. The mutation also limits the capacity of RecA to form long filaments in the presence of ATP. Strains with this mutation do not undergo SOS induc...

The presence of a quinone group in the structure of a series of model compounds was shown to produce cell kill by a mech anism involving free radicals and active oxygen species. Fur thermore, the ability of the compound to bind to DNA appeared to enhance its cytocidal activity. The same model compounds were used to investigate the effect of the quinone group on cellular DNA. DNA single-strand b...

ATF6α and ATF6β are membrane-bound transcription factors activated by regulated intramembrane proteolysis in response to endoplasmic reticulum (ER) stress to induce various ER quality control proteins. ATF6α- and ATF6β single-knockout mice develop normally, but ATF6α/β double knockout causes embryonic lethality, the reason for which is unknown. Here we show in medaka fish that ATF6α is primaril...

Trinucleotide repeat expansions are responsible for more than two dozens severe neurological disorders in humans. A double-strand break between two short CAG/CTG trinucleotide repeats was formerly shown to induce a high frequency of repeat contractions in yeast. Here, using a dedicated TALEN, we show that induction of a double-strand break into a CAG/CTG trinucleotide repeat in heterozygous yea...

Using methods involving transplantation of neuroectoderm and eye rudiments between ap/ap mutants and +/+ embryos of Xenopus laevis, new aspects of melanogenic induction have been ascertained. The possibility of separate induction of a melanocyte cell type and of the product of terminal differentiation in it-melanosomes, has been demonstrated. Melanogenic induction starts at the late gastrula st...

Abstract A time-of-flight Bragg-edge neutron transmission imaging was used to investigate the microstructure and strain distributions in a gear hardened by newly developed two-step induction-heating method: precursor (Sample 1) final product 2). The edge-position edge-broadening were determined mapped with high spatial resolution, which enabled us confirm two-dimensional of residual strain. dee...

Based on the analysis of the structure of the double pinion electric power steering system(DP-EPS),the power steering system with induction motor DP-EPS is modeled and simulated by using the principle of dynamics. By vector control, the induction motor is equivalent to the form of DC motor, which reduces the complexity of EPS control, and lays the foundation for the follow-up control strategy r...

Poly-d-lysine of high molecular weight enhances interferon induction in mice by the double-stranded complex of polyinosinic and polycytidylic acids and is superior to diethylaminoethyl-dextran in this respect.

We propose a Poisson-Lie analog of the symplectic induction procedure, using an appropriate Poisson generalization of the reduction of symplectic manifolds with symmetry. Having as basic tools the equivariant momentum maps of Poisson actions, the double group of a Poisson-Lie group and the reduction of Poisson manifolds with symmetry, we show how one can induce a Poisson action admitting an equ...