Escherichia coli BL21 (DE3) and its derivatives are widely used for the production of recombinant proteins, but these purified proteins are always contaminated with lipopolysaccharide (LPS). LPS is recognized by the toll-like receptor 4 and myeloid differentiation factor 2 complex of mammalian immune cells and leads to release of pro-inflammatory cytokines. It is a vital step to remove LPS from...

BACKGROUND 2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in human milk which protects against the infection of enteric pathogens. Because 2-FL can be synthesized through the enzymatic fucosylation of lactose with guanosine 5'-diphosphate (GDP)-l-fucose by α-1,2-fucosyltransferase (FucT2), an 2-FL producing Escherichia coli can be constructed through overexpressing genes codin...

In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Con...

In this paper optimal control algorithms for two E. coli fed-batch fermentations are developed. Fed-batch fermentation processes of E. coli strain MC4110 and E. coli strain BL21(DE3)pPhyt109 are considered. Simple material balance models are used to describe the E. coli fermentation processes. The optimal feed rate control of a primary metabolite process is studied and a biomass production is u...

In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells. This metabolic burden analysis was performed against a number of recombinant expression systems and control strains and included: (i) standard transcriptional recombinant expression control system BL...

Laccases are multi-copper oxidases that widely distributed in plants and fungi. These enzymes catalyze oxidation of various compounds including phenolics and non-phenolics, and have been used in many industrial processes such as paper industry, biobleaching and bioremediation. A full length cDNA (1767 bp) encoding a putative laccase (Lac) from Rigidoporus vinctus was cloned by polymerase chain ...

One-third of eukaryotic proteins are associated with membranes and these membrane proteins (MP) represent approximately 50% of pharmacological targets. Researchers are therefore nursing the hope of achieving a higher percentage of about 80% in the near future. The only bottleneck to achieving this target is the experimental challengers associated with the production, purification and crystalliz...

Introduction: Alpha-Momorcharin (α-MMC) is a member of the ribosome-inactivating protein (RIP) family that has been widely used as an antitumor, antiviral and antifungal agent. Methods: In this study, codons DNA encoding α-MMC were optimized for expression in E. coli cloned into pET-28a(+) vector. The was then expressed strain BL21 (DE3) purified by nickel affinity chromatography. Results: Unde...

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The tumor suppressor p53 is inactivated by mutation in about half of all tumors, making mutant a prime target for cancer therapy. Missense mutations R248W, R273H and R248Q are included the five most frequent total lead to more than 630 000 new diagnosed cases diseases world every year. These mutations, located at or near protein-DNA interface, inactivation loss direct p53-DNA interactions, caus...