A highly specific, high throughput-amenable bacterial biosensor for chemically induced cellular oxidation was developed using constitutively expressed redox-sensitive green fluorescent protein roGFP2 in E. coli (E. coli-roGFP2). Disulfide formation between two key cysteine residues of roGFP2 was assessed using a double-wavelength ratiometric approach. This study demonstrates that only a few min...

Antimicrobial resistance is a global threat to livestock, human and environmental health. Although resistant bacteria have been detected in wildlife, their role in the epidemiology of antimicrobial resistance is not clear. Our objective was to investigate demographic, temporal and climatic factors associated with carriage of antimicrobial resistant Escherichia coli in raccoons and the environme...

The objective of this study was to analyse the influence of selected factors in relation to total bacterial counts (TBC) in bulk milk samples. Milk samples were tested in nine cowsheds of seven farms for a period of one year. The TBC values were determined by the automated estimation of bacterial counts in cow’s raw milk by directly counting the bacterial cells on a BactoScan 8000 apparatus. Th...

Necrotic enteritis (NE) is an important intestinal infectious disease of commercial poultry flocks caused by Clostridium perfringens. Using an experimental model of NE involving co-infection with C. perfringens and Eimeria maxima, transcriptome profiling and functional genomics approaches were applied to identify the genetic mechanisms that might regulate the host response to this disease. Micr...

Infection of chickens with Eimeria maxima induces the production of parasite-specific antisera which can be used passively to protect naive chickens against infection. Globulin fractions of these antisera can also be used passively to protect chickens. Similarly, intramuscular injection of soybean lectin affinity purified gametocyte antigens of E. maxima in Freund's Complete Adjuvant induces pr...

Objective(s) This study planned to assess antidepressant like activity of aqueous extract from leaves of Citrus maxima Merr. (Rutaceae). Materials and Methods Boiling was used for aqueous extraction. Acute toxicity study was performed in mice. Antidepressant activity was studied using locomotor activity test, modified forced swimming test (FST) and tail suspension test (TST). Three doses 100...

Although Salmonella spp., Escherichia coli O157, Listeria monocytogenes, Enterococcus faecalis and Clostridium spp. present a significant food safety and/or spoilage issue for the beef sector, there are limited data on their prevalence in Irish cattle. The objectives of this preliminary study were to investigate distribution (percentage farms positive) E. L. overall (%) these bacteria cattle sm...

Ionospheric two-stream waves and gradient-drift waves nonlinearly drive a large-scale (D.C.) current in the E-region ionosphere. This current ows parallel to, and with a comparable magnitude to, the fundamental Pedersen current. The underlying mechanism is quite simple. E-region plasma waves generate oscillating electric elds causing electrons to ~ E ~ B drift perpendicular to the wave's propag...

Institute of Organic Chemistry and Biochem Republic, Gilead & IOCB Research Center, Czech Republic. E-mail: [email protected] Institute of Biophysics, v.v.i. Academy of Scie 135, 61265 Brno, Czech Republic. E-mail: fo Central European Institute of Technology, M 625 00 Brno, Czech Republic Department of Organic Chemistry, Faculty Hlavova 8, CZ-12843 Prague 2, Czech Repu † Electronic supplementar...

Elucidation of the molecular details of the cyclic actomyosin interaction requires the ability to examine structural changes at specific sites in the actin-binding interface of myosin. To study these changes dynamically, we have expressed two mutants of a truncated fragment of chicken gizzard smooth muscle myosin, which includes the motor domain and essential light chain (MDE). These mutants we...