The separation of tetracyclines (TCs) using reversed-phase liquid chromatography (LC) is proposed. The use of an amide-based stationary phase prevents the interaction of tetracyclines with the residual silanol groups and thus avoids the appearance of tailed peaks. Detection was based on using an UV spectrophotometer and gradient elution with acetonitrile-oxalic acid as mobile phase permitted go...

Yield and quality are fundamental features for any researchers during nucleic acid extraction. Here, we describe a simplified, semi-unified, effective, and toxic material free protocol for extracting DNA and RNA from different prokaryotic and eukaryotic sources exploiting the physical and chemical properties of nucleic acids. Furthermore, this protocol showed that DNA and RNA are under triple p...

The aim of the study was to evaluate influence 17% ethylenediaminetetraacetic acid (EDTA) and ethanolic extract Sapindus mukorossi (S. mukorossi) in combination with canal shaping on surface properties ProTaper Gold rotary endodontic file. Sixteen F1 files underwent standard protocol for root treatment using two irrigants EDTA) single-rooted decoronated teeth. Eight unused were used as a contro...

The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mutations and to determine the effects of RNA extraction methods, anticoagulants,...

Methods This study describes the molecular characterization of 15 Pseudomonas aeruginosa strains, isolated from endoscopic pus specimens, obtained from sinusitis cases. Susceptibility testing was performed by disk diffusion assay. The MIC of ceftazidime was determined by E test. Ceftazidime (CDM) EDTA combined disc test was used to screen for class B b lactamase production. Plasmid DNA extracti...

We describe a novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme. Heating at 75 degrees C for 15 min inactivates the enzyme before radioimmunoassay. Alternatively, EDTA may be used to in...

Abstract The wide use of copper (Cu)-based fungicide has caused a stepwise accumulation Cu in the environment increasing occurrence phytotoxicity crops. To understand and alleviate this abiotic stress, maize seedlings were grown hydroponic solution with different combinations iron (Fe) forms. Results showed that sensitivity is related to nature form supplied chelate-exchange processes might inv...