Abstract Study question How do compensated egg donors in two different cultural and regulatory settings view anonymous donation understand the implications of consumer ancestry testing? Summary answer Spanish U.S. differ their desire for anonymity, awareness testing, testing maintaining anonymity. What is known already In literature, many have expressed concern that without promise anonymity pe...

background and objectives: hepatitis e virus (hev) is a major public health concern in developing countries. hev transmission occurs primarily by the fecal-oral route. it has also been reported that blood donors are potentially able to cause transfusion-associated hepatitis e in endemic areas. this study aimed to determine the seroprevalence of hev infection among volunteer blood donors in cent...

The CRISPR/Cas9 system is a simple and powerful tool for genome editing in various organisms including livestock animals. However, the system has not been applied to poultry because of the difficulty in accessing their zygotes. Here we report the implementation of CRISPR/Cas9-mediated gene targeting in chickens. Two egg white genes, ovalbumin and ovomucoid, were efficiently (>90%) mutagenized i...

Transfusion of red blood cells (RBCs) into patients with anti–donor RBC antibodies (crossmatch-incompatible transfusion) can result in lethal antibody-mediated hemolysis. Less well appreciated is the ability of anti-RBC antibodies to specifically remove their target antigen from donor RBCs without compromising cell survival or adversely affecting the transfusion recipient. In an effort to eluci...

In our first experiment, we studied the effect of injection method of blastoderm cells (BC) into the subgerminal cavity of White Leghorn embryos on hatchability of chicken chimeras. Freshly laid eggs were injected through a hole made in the equatorial plane of the eggshell (Method A). In Method B, eggs were stored pointed end down for 5 to 7 d prior to injection, and a hole was cut in the blunt...

The use of donor oocytes has expanded the scope of assisted reproductive technology (ART) for women with poor oocyte quantity and quality. In vitro fertilisation with oocyte donation (IVF-OD) is considered to give better implantation, pregnancy, and livebirth rates compared to IVF with autologous oocytes. Maternal age, infertility factors, BMI, smoker status, and ethnicity reduce reproductive o...