zucchini yellow mosaic virus (zymv) causes high yield losses to cucurbits in many parts of the world. during a survey in summer and fall of 2008 and 2009, 392 collected from different fields in khorasan razavi, northern khorasan and southern khorasan provinces were tested based on das-elisa tests using zymv specific polyclonal antibody. the result showed that 72 out of 392 samples were infected...

AIM The aim was to assess the seroprevalence of B. abortus and Leptospira hardjo in the cattle population of Bihar, this work was carried out. MATERIALS AND METHODS Randomly selected 450 cattle from nine districts of Bihar were serologically screened for antibodies against L. hardjo and B. abortus. DAS-ELISA for leptospira and AB-ELISA for brucella were carried out. Based on the results preva...

Infeksi Papaya ringspot virus (PRSV) di Indonesia pertama kali dilaporkan pada pepaya Nangroe Aceh Darussalam tahun 2012. Sejak itu, PRSV atau penyakit bercak bercincin menyebar ke beberapa daerah Jawa, Sumatera, Bali, dan Nusa Tenggara Barat. Penelitian ini dilakukan untuk mengonfirmasi keberadaan penanaman Jawa mengetahui identitas molekulernya. Metode double antibody sandwich enzyme-linked i...

Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) in woody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts were used as template for cDNA synthesis by reverse transcripti...