Enamelin (ENAM) has three putative phosphoserines (pSers) phosphorylated by a Golgi-associated secretory pathway kinase (FAM20C) based on their distinctive Ser-x-Glu (S-x-E) motifs. Fam20C-knockout mice show severe enamel defects similar to those in the Enam-knockout mice, implying an important role of the pSers in ENAM. To determine the role of pSer55 in ENAM, we characterized ENAMRgsc514 mice...

Local periodontal therapy primarily consists of supraand subgingival debridement, and instruction and motivation for the improvement of oral hygiene. Effective regular mechanical plaque control has been proven to be one of the most important measures to avoid and control recurrence of infection (Axelsson and Lindhe, 1978; Axelsson and Lindhe, 1981a; Axelsson and Lindhe, 1981b). In addition resi...

BACKGROUND A number of surgical procedures are effective in covering denuded root surfaces. The first paper in this series evaluated the subepithelial connective tissue graft and the coronally advanced flap with enamel matrix derivative (EMD). That paper revealed no significant difference in the percent of root coverage between the two treatments (P = 0.82). There is limited human histological ...

BACKGROUND The objective of this multicenter, randomized trial was to compare enamel matrix derivative (EMD; test) with barrier membranes (control) for the treatment of mandibular buccal Class II furcation defects. METHODS Forty-five patients with 90 comparable defects on contralateral molars were included. Defects were randomly assigned to EMD or bioabsorbable barrier membrane; the contralat...

The recession of the gingiva is increasingly becoming a prominent condition in the oral health of many patients and should be treated at its earliest detection. Part I of this discussion reviewed the multifactorial etiology and decision modality; a treatment option was demonstrated for gingival recession using the tunneling technique. This concluding part of the discussion highlights two clinic...

BACKGROUND This split-mouth, double-blind randomized controlled trial evaluated radiographic changes in infrabony defects treated with open flap debridement (OFD) or OFD associated with enamel matrix derivative (EMD) after a 24-month follow-up. The radiographic distance from the CEJ to the bottom of the defect (BD) was considered the primary outcome. CEJ-BC and defect angle were secondary outco...

The efficacy of an oily calcium hydroxide suspension (Osteoinductal) as an adjunct to periodontal regenerative therapy has been demonstrated in recent clinical and histological studies. However, little is known about the molecular mechanisms in vitro, particularly, about the effect of oily calcium hydroxide paste on periodontal ligament (PDL) cells. Therefore the aim of the present study was to...

Aim: To investigate the effect of Emdogain gel (EMD) in pulpotomized primary molars and its clinical and radiographic outcomes. Methods and Materials: In this study, 18 lower second primary molars of nine children were treated by pulpotomy. The teeth were randomly assigned to the EMD (experimental) and Formocresol (control) groups in each patient (split mouth). Following removal of the coronal ...

The recognition that periodontal regeneration can be achieved has resulted in increased efforts focused on understanding the mechanisms and factors required for restoring periodontal tissues so that clinical outcomes of such therapies are more predictable than those currently being used. In vitro models provide an excellent procedure for providing clues as to the mechanisms that may be required...

BACKGROUND Extracellular matrix proteins are key factors that influence the regenerative capacity of tissues. The objective of the present study was to evaluate the effects of enamel matrix derivative (EMD), TGF-β1, and the combination of both factors (EMD+TGF-β1) on human osteoblastic cell cultures. METHODS Cells were obtained from alveolar bone of three adult patients using enzymatic digest...