During a 2009 nationwide outbreak of sorbitolfermenting Escherichia coli O157 in Norway, the Norwegian Institute of Public Health was notified of diarrhoea outbreaks in two nurseries. A link to the nationwide outbreak was suspected and investigated, including retrospective cohort studies. Both nurseries had recently visited farms. Faecal specimens were obtained from symptomatic children as well...

The bovine gastrointestinal tract is the main reservoir for enterohaemorrhagic Escherichia coli (EHEC) responsible for food-borne infections. Characterization of nutrients that promote the carriage of these pathogens by the ruminant would help to develop ecological strategies to reduce their survival in the bovine gastrointestinal tract. In this study, we show for the first time that free ethan...

This study aimed to evaluate the microbiological quality of water four ponds used for irrigation on Lagoa do Sino Farm, as well perform genotypic characterization virulence factors in Escherichia coli isolates. Sampling was conducted 11 months, between 2015 and 2016. Samples were analyzed presence thermotolerant coliforms, E. heterotrophs. DNA extracted from isolates, followed by polymerase cha...

BACKGROUND Several cases of human infection caused by verotoxin-producing Escherichia coli (VTEC) O157:H7 in Sweden have been connected with cattle farm visits. Between 1996 and 2002, 18 farms were classified as the source of human cases with isolation of EHEC (Enterohaemorrhagic Escherichia coli) after VTEC O157:H7 had been isolated from cattle on those farms. RESULTS Characterization by pha...

Transcription of the LEE1 operon in the locus of enterocyte effacement of enterohaemorrhagic Escherichia coli is due to the P1 promoter. Mutational and biochemical analyses reveal the existence of an overlapping promoter, designated P1A, which can drive transcript initiation 10 bp upstream of the P1 promoter transcript start point. Because of the overlap between P1 and P1A, P1A activity is unma...

background: extended spectrum beta lactamases (esbls) are an important cause of transferable multidrug resistance (mdr) in gram-negative bacteria. the most described esbl genes are generally found within integron-like structures as mobile genetic elements. the aim of this study was to identify the accompanying of class 1 integrons and esbls in the mdr e. coli isolates. methods: susceptibility t...

Enterohaemorrhagic Escherichia coli (EHEC) is a major foodborne pathogen and tightly adheres to human colonic epithelium by forming attaching/effacing lesions. To reach the epithelial surface, EHEC must penetrate the thick mucus layer protecting the colonic epithelium. In this study, we investigated how EHEC interacts with the intestinal mucus layer using mucin-producing LS174T colon carcinoma ...