The use of liposomes as carriers of therapeutic agents has been extensively reviewed (Tyrrell et al., 1976). We (Patel & Ryman, 1976) have shown that encapsulation within liposomes may be an effective way of administering insulin orally, by which route it is normally destroyed in the gastrointestinal tract. When liposomally entrapped insulin was given orally to diabetic rats, there was a signif...

A novel bienzyme-channeling sensor was constructed by entrapping glucose oxidase (GOD) and horseradish peroxidase (HRP) in the mesopores of well-ordered hexagonal mesoporous silica structures (SBA-15). The SBA-15 mesoporous materials accelerated the electron transfer between the entrapped HRP and electrode. Both HRP and GOD retained their catalytic activities in the bienzyme-entrapped SBA-15 fi...

Efficient functioning of enzymes inside liposomes would open new avenues for applications in biocatalysis and bioanalytical tools. In this study, the entrapment of amyloglucosidase (AMG) (EC 3.2.1.3) from Aspergillus niger into dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles (MLVs) and large unilamellar vesicles (LUVs) was investigated. Negative-stain, freeze-fracture, and cryo-tra...

The paper describes development of a sol-gel based immunoaffinity method for the steroid hormone levonorgestrel (LNG) and the effects of changes in the sol-gel matrix format on the activity of the entrapped antibodies (Abs) and on matrix structure. The best sol-gel format for Ab entrapment was found to be a tetramethoxysilane (TMOS) based matrix at a TMOS:water ratio of 1:8, containing 10% poly...

TAKADA, G., ONODERA, II. and TADA, K. Tissue Distribution of Unentrapped or Liposome-Entrapped 1311-Labeled f3-Galactosidase Injected into Rats. Tohoku J. exp. Med., 1981, 134 (2), 103-114 Either unentrapped (free) or liposomeentrapped 131I-labeled 9-galactosidase was injected into rats from tail veins. Tissue distribution and intracellular localization of the radioactivity of both sources were...

Attempts have been made to optimize immobilization parameters, catalytic property, and stability of immobilized α-amylase in agar. The work compares natural entrapment efficiency of agar with the ionotropically cross-linked agar hydrogel, with the advantage of easy scale-up and cost and time effectiveness. Beads prepared with 3% (w/v) agar and 75 mM calcium chloride and hardened for 20 minutes ...

abstract biofertilizers are one of the main sources to supply nutrient elements for plants in sustainable agriculture. in order to evaluate the effects of biofertilizers and chemical fertilizers on cumin (cuminum cyminum) as a medicinal plant a field experiment was conducted in the research farm of faculty of agriculture, ferdowsi university of mashhad in a randomized complete block design with...

to study the effects of biofertilizers in their different levels of nitrogen (n) and phosphorus (p) on yield related parameters of barley (sahra cultivar), a split plot experiment based upon a randomized complete block design of three replications was conducted. main plots consisted of: nitrogen at three levels (0, 50 and 100 kg n ha-1 as urea), while sub plots were: phosphorus at two levels (0...

The formation of an excess of dissolved gas (“excess air”) in quasi-saturated media was studied by analyzing and interpreting dissolved noble gas concentrations in laboratory column experiments. Using quartz sand filled columns of 1 m length, two different experimental designs were realized. In the first, groundwater recharge was simulated by a unidirectional vertical water flow through the col...