Ribonuclease A has been the most studied enzyme of the twentieth century. Work on ribonuclease A has endowed enzymology, as well as protein science, with many paradigms. This work continues to provide a framework for understanding protein structure – function relationships in atomic detail. In addition, knowledge gained by basic research is enabling the rapid deployment of ribonuclease A homolo...

* Supported in part by a grant from the Atomic Energy Commission, Contract No. AT (30-1) 1017. t Public Health Research Fellow of the National Cancer Institute. 1 Yanofsky, C., J. Biol. Chem., 223, 171 (1956). 2 Yanofsky, C., and M. Rachmeler, Biochim. et Biophys. Acta, 28, 640 (1958). 3 DeMoss, J. A., M. Imai, and D. M. Bonner, Bact. Proc., 112 (1958). 4Yanofsky, C., Biochem. et Biophys. Acta,...

the present investigation was aimed at developing a novel colon targeted system of lornoxicam based on the use of a combination of ph dependent system (to prevent the premature release of drug in the upper git) and enzymatically degradation system (to ensure the specificity of drug release in the colon). the drug loaded guar gum microspheres prepared by emulsification cross-linking method were ...

Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from starch industry, were used as carbon nitrogen sources, respectively, for lipid production, using the heterotrophic microalga C. cohnii grown in bench bioreactor, batch culture. The maximum biomass concentration, content productivity attained 5.34 g/L, 24.6% (w/w Dry Cell Weight-DCW) 0.016 g L−1 h−1, respect...

Objective(s): Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. Materials and Methods: The enzymatic region of hyaluronidase gene was detecte...

With the advent of flexible electronics, old fashioned and conventional solid-state technology will be replaced by conductive inks combined with low-cost printing techniques. Graphene is an ideal candidate to produce inks, due its excellent conductivity zero bandgap. The possibility chemically modify graphene active molecules opens up field responsive inks. Herein, a bioresponsive, electroactiv...

Copoazú is a fruit originated from Colombian Amazon belonging to Theobroma genus, which includes species such as cacao raw material for chocolate. Both presented similar processing stages, with different process parameters due the physical qualities of each fruit. Enzymatic, mechanical, and combined methods pulping copoazú seeds their effect on biochemical dynamics fermentation were evaluated. ...

mucoadhesive polymers that bind to the gastric mucin or epithelial cell surface are useful in drug delivery for the purpose of increasing the intimacy and duration of contact of drug with the absorbing membrane. several synthetic polymers are in use for this purpose. since the biodegradability of the synthetic polymers are questionable, in this investigation, an oral mucoadhesive controlled del...

long protocol of gonadotropin-releasing hormone-analougue (gnrh-a) can result in the formation of ovarian cyst by the transient initial stimulatory effect which increases the levels of both follicle-stimulating hormone (fsh) and luteinizing hormone (lh). these cysts require surgical drainage or result in poor ovarian response. ovarian cyst formation can be prevented by taking oral contraceptive...