A double labeling procedure was employed to determine whether the tyrosine residues in thyroglobulin that are iodinated first are also those that are most readily converted to thyroxine. Goiter thyroglobulin containing less than 1 atom of iodine per molecule was iodinated first with 9 at relatively low levels of iodination (5 to 20 atoms of iodine per molecule) and afterward with lzjI at higher...

A case of endophthalmitis due to caterpillar setae was, for the first time, successfully treated by surgical removal of the setae. The procedure was facilitated by the use of sodium hyaluronidate. Scanning and transmission electron microscopy of the setae showed evidence of enzymatic degradation of the shaft and burial of the spines in exudate.

in current study, protein hydrolysates were produced using microbial proteases of alcalase, protamex and flavourzyme from yellowfin tuna viscera. the results indicated that, protein hydrolysate from alcalase, had significantly higher protein content, nitrogen recovery and degree of hydrolysis than the others (p 0.05). the results of amino acids composition revealed that all hydrolysates were al...

esterification of chitosan with adipic acid catalyzed by immobilized candida antarctica lipase b was carried out in this study. response surface methodology (rsm) based on a four-factor- five-level small central composite design (sccd) was employed to model and analyze the reaction. a total of 21 experiments representing different combinations of the four reaction parameters including ch...

Mathematical modeling is an indispensable tool for research and development in biotechnology and bioengineering. The formulation of kinetic models of biochemical networks depends on knowledge of the kinetic properties of the enzymes of the individual reactions. However, kinetic data acquired from experimental observations bring along uncertainties due to various experimental conditions and meas...

the etiopathogenesis of alzheimer's disease (ad) is still unclear.  however, long-term oxidative stress is believed to be one of the major contributing factors in progression of neuronal degeneration and decline of cognitive function in ad. in order to assess the presence of oxidative stress in ad, we examined the enzymatic activities of the erythrocyte cu-zn superoxide dismutase (cu-zn sod), g...

Fractal modifications of Fick?s laws are discussed by taking into account the electrode?s porous structure, and a fractal derivative model for diffusion-reaction process in thin film an amperometric enzymatic reaction is established. Particular attention paid to giving intuitive grasp its variational principle solution procedure. Extremely fast or extremely slow diffusion can be achieved suitab...