The ecto-5′-nucleotidase CD73 is involved in the production of immunosuppressive adenosine tumoral microenvironment and recently became a validated target immuno-oncology. To avoid formation CD73-produced adenosine, several series potential inhibitors enzyme based on triazole scaffold were synthetized evaluated recombinant purified hCD73 cell-based assays.

In vitro evaluation of enzyme supplementation is used to predict the in vivo response poultry enzymes. This study aims evaluate efficacy techniques predicting supplementation. Different such as pepsin, amylase, and protease assays were efficacy. The results obtained from compared with same showed that can be effectively recommends use for