In this study we characterized phosphoribulokinase (PRK, EC 2.7.1. 19) from the eukaryotic marine chromophyte Heterosigma carterae. Serial column chromatography resulted in approximately 300-fold purification of the enzyme. A polypeptide of 53 kD was identified as PRK by sequencing the amino terminus of the protein. This protein represents one of the largest composite monomers identified to dat...

Chloramphenicol acetyltransferase [acetyl-CoA:chloramphenicol O3-acetyltransferase; EC 2.3.1.28] is the enzyme responsible for high-level bacterial resistance to the antibiotic chloramphenicol. It catalyzes the transfer of an acetyl group from acetyl CoA to the primary hydroxyl of chloramphenicol. The x-ray crystallographic structure of the type III variant enzyme from Escherichia coli has been...

Microreaction technology, which is an interdisciplinary science and engineering area, has been the focus of different fields of research in the past few years. Several microreactors have been developed. Enzymes are a type of catalyst, which are useful in the production of substance in an environmentally friendly way, and they also have high potential for analytical applications. However, not ma...

A simple and efficient synthesis of aryl and heteroaryl substituted pyrimidines has been developed via initial Knoevenagal, subsequent addition and final cyclization of aldehyde, ethylcyanoacetate and guanidine nitrate. Piperidine has been used as a catalyst. Short reaction time, environment friendly procedure and excellent yields are the advantages of this procedure. All synthesized compounds ...

The aim of this paper is to analyze how different catalyst activity profiles influence the operating strategies of industrial reactors. Based on this analysis a method that can be used to transform information given by laboratory reactor experiments into a form which can be used to estimate the productivity of the catalyst and the quality properties of polymers under industrial conditions is pr...

Xylanases from family 10 glycanases contain three conserved histidine residues in their active site. The role of H86 in the structure-function of xylanase A from Streptomyces lividans (XlnA) was studied by site-directed mutagenesis. Six mutant proteins (H86A/E/F/K/Q/W) were produced, purified and characterized. The six mutations reduced the affinity of XlnA towards xylan without having any majo...

Sequence alignment and modeling of the 2-kinase domain of the liver bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase, on 6-phosphofructo-1-kinase from Bacillus stearothermophilus and Escherichia coli (Bazan, J. F., Fletterick, R. J., and Pilkis, S. J. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9642-9646) suggested that Cys-160 of the 2-kinase would correspond to Asp-127 ...

Peroxidase enzymes are crucial in analytical chemistry owing to significant peroxide analytes and their key role hydrogen (H2O2) detection. Therefore, exploiting appropriate catalysts for the peroxidase like reactions has become achieving desired performance. Zirconium (Zr) attracted growing interest, as a safe stable potential eco-friendly catalyst various organic transformations that address ...