The use of enzymes in feedlot diets has not been evaluated extensively, and most research with high-concentrate diets has been with barley. Beauchemin et al. (1997) evaluated the addition of two enzyme mixtures that varied in the proportion of xylanase and cellulase activity to 95.1% concentrate feedlot diets based on either barley or corn. Feed efficiency for the overall finishing period was i...

Enzymes have been widely studied for their excellent efficiency, selectivity and environmentally benign nature. However, the development of biocatalytic applications is severely limited by lack stability reusability. Nanostructured materials demonstrated as efficient hosts immobilization enzyme due to large surface areas well higher activities long-term stability. Furthermore, nanomaterials wit...

Ervatamins (A, B and C) are papain-like cysteine proteases from the plant Ervatamia coronaria. Among Ervatamins, Ervatamin-C is a thermostable protease, but it shows lower catalytic efficiency. In contrast, Ervatamin-A which has a high amino acid sequence identity (∼90%) and structural homology (Cα rmsd 0.4 Å) with Ervatamin-C, has much higher catalytic efficiency (∼57 times). From the structur...

Titanium dioxide (TiO2) and Fe3O4 magnetite particles were coated on spherical Kissirises; glucose oxidase (GOx) enzyme was immobilized on Kissiris/Fe3O4/TiO2 by physical adsorption. This catalyst was analyzed by a scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray (EDX) measurements. The performance of the prepared biocatalyst in the...

Nine Aspergillus and three of Trichoderma strains were grown on wheat bran (WB) medium under solid state fermentation (SSF) for amylase production. Aspergillus oryzae LS1 produced the highest level of the enzyme. The thermal stability profile of its crude enzyme revealed the half-life time of more than 2 h at 50 and 60oC. The enzyme production was affected by strain type, incubation periods, le...

Objective(s): Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. Materials and Methods: The enzymatic region of hyaluronidase gene was detecte...

The biopharmaceuticals market is constantly growing. Despite their advantages over the conventional drugs, have short biological half-lifes, which can be increased using liposomes. However, common bulk methods to produce biopharmaceuticals-loaded liposomes result in lost of encapsulation efficiency (E.E.), resulting an expensive process. Herein, a therapeutic enzyme proposed, glass-capillary mi...