Abstract Sweet sorghum stem dregs have potential as an alternative raw material for bioethanol production due to its high sugar content. The purpose of this study is examine the hydrolysis kinetics sweet with cocktail enzymes using several models, namely Valjamae, Kopelman, and Gompertz models. was carried out at a concentration 1/16.7 g/mL, temperature 50°C, variations time (0, 12, 24, 36, 48,...

The enzymatic membrane reactor (EMR) consists on a coupling of a membrane separation process with an enzymatic reaction. The selective membrane aims to separate the biocatalyst from the reaction products. The main objective of EMR is to ensure the complete rejection of the enzyme in order to maintain the full activity inside the reacting volume. Depending on the case, enzyme molecules may be fr...

Enzyme-mediated oxidation is of particular interest to synthetic organic chemists. However, the implementation of such systems demands knowledge of enzyme kinetics. Conventionally collecting kinetic data for biocatalytic oxidations is fraught with difficulties such as low oxygen solubility in water and limited oxygen supply. Here, we present a novel method for the collection of such kinetic dat...

The thermal deactivation of TEM-1 β-lactamase was examined using two experimental techniques: a series of isothermal batch assays and a single, continuous, non-isothermal assay in an enzyme membrane reactor (EMR). The isothermal batch-mode technique was coupled with the three-state "Equilibrium Model" of enzyme deactivation, while the results of the EMR experiment were fitted to a four-state "m...

A rapid and economical method is reported for the preparation of an immobilized enzyme reactor (IMER) using silica-encapsulated equine butyrylcholinesterase (BuChE) as a model system. Peptide-mediated silica formation was used to encapsulate BuChE, directly immobilizing the enzyme within a commercial pre-packed column. The silica/enzyme nanocomposites form and attach simultaneously to the metal...

Enzymatic hydrolysis of lactose by -galactosidase (Lactozym) has been studied in a jacketed stirred-tank reactor (JSTR) and in a recirculation hollow-%bre bioreactor (RHFB). The hollow-%bre module was manufactured with modi%ed cellulose-acetate membrane (Cuprophan). The variables tested were: temperature (25–40◦C), lactose concentration (0.0278–0:208 M) and enzyme concentration (0.100–4:51 g l−...

Fish proteins are found in the flesh, head, frames, fin, tail, skin and guts of the fish in varying quantities. Unutilized fish and fish processing waste can be used to produce fish proteins which contain amino acids and many bioactive peptides. After removing the fish flesh during the fish processing operation, all other parts are considered wastes which are not properly utilized. The aim of t...

the steps taken in designing a loop reactor for bulk propylene polymerization are discussed. the roles played by several parameters like particle growth factor, catalyst activity and solid fraction on reactor volume at different operating conditions are investigated. at first, based on a fixed production capacity and specific rate of catalyst, reactor volume is determined. next, the length and ...

−− The synthesis of ethyl hexadecanoate was carried out by esterification of palmitic acid with ethanol in a solvent-free system. A commercial immobilized lipase (Lipozyme RM-IM) was mixed with the reagents, in a 15 mL closed batch reactor with constant stirring and coupled with a condenser. The effects of palmitic acid/ethanol molar ratio (0.16 to 1.84), reaction temperature (65 to 75C) and en...