ObjectiveMetabolomic profiling of seminal plasma has been suggested as a possible approach for fast and non-invasive male infertility evaluation diagnosis. However, metabolomics profiles in normozoospermic men have not thoroughly investigated, the influence ejaculation-abstinence described.To provide interim reference values find associations between human length period men.Study designSemen sa...

Scientific relevance . Endocrine disruptors affect the functioning of endocrine organs, which leads to adverse drug reactions. toxicity requires special attention in preclinical studies candidate medicinal products. Aim. The study aimed review international guidelines and approaches assessing risk associated with Discussion. This covers documents that provide a methodological framework for iden...

BACKGROUND The Sperm Adhesion Molecule 1 (SPAM1) is an important sperm surface hyaluronidase with at least three functions in mammalian fertilization. Previously our laboratory reported that in the mouse, in addition to its expression in the testis, Spam1 is synthesized in the epididymis where it is found in membranous vesicles in the principal cells of the epithelium in all three regions. Sinc...

cyclophosphamide (cp) is extensively used as an antineoplastic agent for the treatment of various cancers, as well as an immunosuppressive agent. however, despite its wide spectrum of clinical uses, cp is known to cause several adverse effects including reproductive toxicity. crataegus monogyna is one of the oldest pharmaceutical plants that have been shown to be cytoprotective by scavenging fr...

Background The main aim of the present study was to evaluate the ameliorative effect of curcumin on aflatoxin-induced changes in caput and cauda epididymis of mice. MaterialsAndMethods Aflatoxin was obtained by growing Aspergillus parasiticus in sucrose - magnesium sulphate - potassium nitrate - yeast extract (SMKY) liquid medium. Pure curcumin (97% purity) was purchased from Hi-Media Laborator...

Mouse cauda epididymis were in-vivo transfected using the lipid FuGENE 6 as gene vector. Two gene constructions were employed: the p-GeneGRIP which codifies for the Green Fluorescent Protein (GFP) and the pSEAP-control that expresses an alkaline phosphatase as a secretion. Transfection was detected by fluorescence and appeared in the nucleus and cytoplasm of epithelial cells. Transfection was o...

Cryopreservation of caudal epididymal spermatozoa is an effective technique to conserve genetic potentials of superior dogs when it is not possible to collect ejaculated spermatozoa. Although hen egg yolk is commonly supplemented into the semen extender, active substances within the egg yolk which protect sperm against cryoinjury remain to be discovered. Among its compositions, low-density lipo...

Therapeutic targets for male contraception are associated with numerous problems due to their focus on disrupting spermatogenesis or hormonal mechanisms to produce dysfunctional sperm. Here we describe the dual genetic deletion of α1A-adrenergic G protein-coupled receptors (adrenoceptors) and P2X1-purinoceptor ligand gated ion channels in male mice, thereby blocking sympathetically mediated spe...