The detection rate of CTX-M-type β-lactamase-producing Enterobacteriaceae in Japan has significantly increased. Nursing homes may be a reservoir of antibiotic-resistant bacteria. Therefore, we determined the prevalence of, and risk factors associated with, fecal carriage of CTX-M-type β-lactamase-producing Enterobacteriaceae among nursing home residents. A total of 225 stool samples were collec...

OBJECTIVES To characterize the prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6')-Ib-cr in extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. METHODS qnrA, qnrB, qnrS, aac(6')-Ib-cr and ESBL-encoding genes were detected by PCR. MICs of 10 antimicrobial agents were determined by Etest. PFGE was used to investigate the clo...

BACKGROUND Escherichia coli is an important bacterial species based on incidence and associated infection severity. Some E. coli strains produce extended-spectrum beta lactamase (ESBL) and are called ESBL-producing E. coli. These strains are resistant to most classes of cephalosporin and a number of other classes of antibiotics. Plasmids carrying qnr genes have been found to transmit quinolone ...

OBJECTIVES To characterize the naturally occurring β-lactamase gene identified from a clinical isolate belonging to a novel enterobacterial species that is closely related to Rahnella spp. and Ewingella spp. METHODS Shotgun cloning and expression in Escherichia coli were performed in order to characterize this resistance determinant. Enzymatic activities were measured by UV spectrophotometry ...

conclusions according to our results, blatem-116 was the most frequent isolated esbl gene among the p. aeruginosa strains isolated from patients. results ciprofloxacin and piperacillin were the most efficient antipseudomonal agents. the results disclosed that 19 (16.37%) of the isolates were multidrug resistant and 8 (6.89%) were esbl-positive. of the 116 isolates, 30 (25.86%) were resistant to...

objective(s): drug resistant acinetobacter baumannii have emerged as a major problem in many hospitals and intensive care units. various types of extended spectrum beta-lactamases (esbls) are responsible for resistance to beta- lactam antibiotics in different parts of the world. the objective of this study was to determine the prevalence of integron class1 (int 1) and esbl types per-1, per-2 an...

Escherichia coli is generally considered as a commensal inhabitant of gastrointestinal tract of humans and animals. The aim of this study was to gain insight on the distribution of phylotypes and presence of genes encoding integrons, extended β-lactamases and resistance to other antimicrobials in the commensal E. coli isolates from healthy adults in Chandigarh, India. PCR and DNA sequencing wer...

The extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli clone ST131 (ESBL-ST131) has spread in healthcare settings worldwide. The reasons for its successful spread are unknown, but might include more effective transmission and/or longer persistence. We evaluated the colonisation dynamics of ESBL-producing E. coli (ESBL-EC), including ESBL-ST131, in a long-term care facility (LTCF...