نتایج جستجو برای: escherichia coliecoli

تعداد نتایج: 126679  

2015
Lothar Beutin Sabine Delannoy Patrick Fach Pina Fratamico

Enterohemorrhagic E. coli (EHEC) serogroup O145 is regarded as one of the major EHEC serogroups involved in severe infections in humans. EHEC O145 encompasses motile and non-motile strains of serotypes O145:H25 and O145:H28. Sequencing the fliC-genes associated with the flagellar antigens H25 and H28 revealed the genetic diversity of the fliCH25 and fliCH28 gene sequences in E. coli. Based on a...

Journal: :Journal of clinical microbiology 2008
Chase E Guion Theresa J Ochoa Christopher M Walker Francesca Barletta Thomas G Cleary

Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical. We developed a real-time fluorescence-based multiplex PCR for the detection of al...

2015
Thando Ndlovu Marcellous Le Roux Wesaal Khan Sehaam Khan

McNemar's test and the Pearson Chi-square were used to assess the co-detection and observed frequency, respectively, for potentially virulent E. coli genes in river water. Conventional multiplex Polymerase Chain Reaction (PCR) assays confirmed the presence of the aggR gene (69%), ipaH gene (23%) and the stx gene (15%) carried by Enteroaggregative E. coli (EAEC), Enteroinvasive E. coli (EIEC) an...

Journal: :Journal of infection in developing countries 2015
Casmir Ifeanyichukwu Cajetan Ifeanyi Nkiruka Florence Ikeneche Bassey Enya Bassey Nazek Al-Gallas Ridha Ben Aissa Abdellatif Boudabous

INTRODUCTION Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown. METHODOLOGY Stool specimens from 400 acute diarrheic children betw...

Journal: :Journal of clinical microbiology 2007
Ji-Rong Yang Fang-Tzy Wu Jin-Lai Tsai Jung-Jung Mu Ling-Fen Lin Kuang-Lo Chen Steve Hsu-Sung Kuo Chuen-Sheue Chiang Ho-Sheng Wu

To compare the diarrheagenic Escherichia coli (DEC) identifications obtained between traditional O serotyping and modern virulence gene detection assays, we developed a multiplex real-time PCR assay by detecting six specific virulence genes for enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC), enterotoxigenic E. coli (ETEC), and enteroinvasive E. coli (EIEC). Among 261 clinical...

Journal: :Diagnostic microbiology and infectious disease 2010
Priya Rajendran Sitara Swarna Rao Ajjampur Divya Chidambaram Gunasekaran Chandrabose Bhuvaneswari Thangaraj Rajiv Sarkar Prasanna Samuel Deva Prasanna Rajan Gagandeep Kang

The prevalence of diarrheagenic Escherichia coli (DEC) in children under 5 years was studied in children with diarrhea and controls in South India. Four polymerase chain reaction (PCR) "schemes" were used to detect genes of the 6 pathotypes of DEC. In 394 children with diarrhea, 203 (52%) DEC infections were found. Among the 198 controls, 126 (63%) DEC infections were found. Enteroaggregative E...

Journal: :Applied and environmental microbiology 2011
W Ahmed L Hodgers N Masters J P S Sidhu M Katouli S Toze

In this study, 200 Escherichia coli isolates from 22 rainwater tank samples in Southeast Queensland, Australia, were tested for the presence of 20 virulence genes (VGs) associated with intestinal and extraintestinal pathotypes. In addition, E. coli isolates were also classified into phylogenetic groups based on the detection of the chuA, yjaA, and TSPE4.C2 genes. Of the 22 rainwater tanks, 8 (3...

Journal: :Advanced healthcare materials 2014
Andrew L Hook Chien-Yi Chang David J Scurr Robert Langer Daniel G Anderson Paul Williams Martyn C Davies Morgan R Alexander

The thermally triggered release of up to 96% of attached uropathogenic E. coli is achieved on two polymers with opposite changes in surface wettability upon reduction in temperature. This demonstrates that the bacterial attachment to a surface cannot be explained in terms of water contact angle alone; rather, the surface composition of the polymer plays the key role.

2013
Gaëtan Muyldermans Amber Litzroth Geneviève Ducoffre Sophie Quoilin

Background: On 5 June 2012 several enterohemorrhagic Escherichia coli, EHEC, O157:H7 infections were reported to the public health authorities of Limburg. Methods: We performed a case-control study, a trace back/forward investigation and compared strains isolated from human cases and food samples. A case was defined as anyone with a laboratory-confirmed E. coli O157:H7-infection in North-East L...

2018
Aixia Xu Sarah Hertrich David S. Needleman Shiowshuh Sheen Christopher Sommers

Uropathogenic Escherichia coli serotype O4:H5 isolates (ATCC 700414, 700415, 700416, and 700417) were recovered from women with first-time urinary tract infections. Here, we report the draft genome sequences for these four E. coli isolates, which are currently being used to validate food safety processing technologies.

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