Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that include oocytes, blastocysts, and embryonic and adult stem cells, we obtained 249,200 high-quality EST sequences and clustered them with public ...

PURPOSE Bioinformatics has emerged as a powerful tool for identifying novel genes and pathways associated with retinal biology and disease. The developing mouse retina expresses an exceedingly large and complex variety of genes. Many of these genes have not been characterized but nevertheless are likely to have important developmental or physiological functions. The purpose of this study was to...

fusarium head blight (fhb) caused by fusarium graminearum is a serious disease of wheat (triticum aestivum l.), through which grain quality losses are induced by fungal trichotecene mycotoxins such as deoxynivalenol (don). a class of plasma membrane localized abc transporter proteins related to the yeast pdr5 (pleiotropic drug resistance5) efflux pump seems to be responsible for partial resista...

The TBestDB database contains approximately 370,000 clustered expressed sequence tag (EST) sequences from 49 organisms, covering a taxonomically broad range of poorly studied, mainly unicellular eukaryotes, and includes experimental information, consensus sequences, gene annotations and metabolic pathway predictions. Most of these ESTs have been generated by the Protist EST Program, a collabora...

A total of 10,154 5'-end expressed sequence tags (EST) were established from the normalized and size-selected cDNA libraries of a marine red alga, Porphyra yezoensis. Among the ESTs, 2140 were unique species, and the remaining 8014 were grouped into 1127 species. Database search of the 3267 non-redundant ESTs by BLAST algorithm showed that the sequences of 1080 species (33.1%) have similarity t...

Advanced studies of apple (Malus domestica Borkh) development, physiology, and biochemistry have been hampered by the lack of appropriate genomics tools. One exception is the recent acquisition of extensive expressed sequence tag (EST) data. The entire available EST dataset for apple resulted from the efforts of at least 20 contributors and was derived from more than 70 cDNA libraries represent...

Advanced studies of apple (Malus domestica Borkh) development, physiology, and biochemistry have been hampered by the lack of appropriate genomics tools. One exception is the recent acquisition of extensive expressed sequence tag (EST) data. The entire available EST dataset for apple resulted from the efforts of at least 20 contributors and was derived from more than 70 cDNA libraries represent...

expressed sequence tags simple sequence repeats (est-ssrs) are important sources for investigation ofgenetic diversity and molecular marker development. similar to genomic ssrs, the est-ssrs are usefulmarkers for many applications in genetics and plant breeding such as genetic diversity analysis, molecularmapping and cross-transferability across related species and genera. in spite of low polym...

A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and...