Endometrial prostaglandins (PGs) and the PGE2/PGF2alpha ratio play an important role in regulating the estrous cycle and establishment of pregnancy. The enzymes downstream of cyclooxygenase-2 may determine the PGE2/PGF2alpha ratio in the porcine uterus. Thus, we have cloned porcine PGF synthase (PGFS) and microsomal PGE synthase-1 (mPGES-1) and characterized their expression in porcine endometr...

Carbofuran, a systemic N-methyl carbamate pesticide was orally administered with the doses of 0.4, 0.7, 1 and 1.3 mg/kg body weight/day to normal virgin female Swiss albino mice for 30 days. The vaginal smear and body weight of mice were recorded daily and mice were sacrificed on the 31st day. Estrous cycle was effected by showing a significant decrease in the number of estrous cycle and the du...

Experiments were designed to test the hypothesis that LHRH receptors in the preoptic area (POA) are of physiological importance for maintaining estrous cyclicity in the rat. Bilateral cannulae were implanted just dorsal to the POA. Estrous cycles were monitored daily by vaginal smears. Antide, a long-acting LHRH antagonist, was infused bilaterally (2.5 micrograms/side) in the POA or the hypotha...

Aim: Present study was undertaken to investigate profiling of prostaglandin F receptor (PGFR) mRNA expression during estrous cycle and early pregnancy. Materials and Methods: Female genitalia of goats (n=3) were collected from a local abattoir. Based on color, vasculature, size, and consistency of corpus luteum (CL), the uteri were classified into three stages of the estrous cycle: stage I: Day...

The aim of the present study was to investigate the distribution and density of noradrenergic nerve fibres (NNFs), content of catecholamines (CATs) and steroids in the cystic ovaries of gilts receiving DXM from middle luteal phase. Cystic status of ovaries was induced by i.m. DXM injections on days 7-21 of the estrous cycle. During the same time, gilts in the control group received saline. The ...

Naghashpour, M., and G. Dahl. Sensitivity of myometrium to CGRP varies during mouse estrous cycle and in response to progesterone. Am. J. Physiol. Cell Physiol. 278: C561–C569, 2000.—Calcitonin gene-related peptide (CGRP) inhibits contractions of the myometrium. Isometric force measurements on myometrial strips were carried out to monitor the inhibitory capacity of CGRP in the myometrium during...

Bisphenol A (BPA) is a monomer used in a polymerization reaction in the production of polycarbonate plastics. It has been used in many consumer products, including plastics, polyvinyl chloride, food packaging, dental sealants, and thermal receipts. However, there is little information available on the inhalation toxicity of BPA. Therefore, the aim of this study was to determine its inhalation t...

In the present study, we investigated the effect of PPAR ligands on progesterone (P4) and 17β-estradiol (E2) secretion, as well as 3β-hydroxysteroid dehydrogenase/Δ(5)-Δ(4) isomerase (3β-HSD) mRNA expression, in porcine endometrial slices collected on days 10–12 and 14–16 of the estrous cycle or early pregnancy. The explants were incubated in vitro for 6 h in the presence of PPARα ligands – WY-...

Ghrelin, a novel motilin-related endogenous ligand for growth hormone secretagouge receptor, is implicated in various biological functions, including regulation of female reproduction. But the presence of ghrelin and its role in reproductive functions in buffalo, a species with poor reproductive efficiency, is not known. In the present study full-length ghrelin cDNA was isolated from bubaline a...

Better breeding strategies for captive Asian elephants in range countries are needed to increase populations; this requires a thorough understanding of their reproductive physiology and factors affecting ovarian activity. Weekly blood samples were collected for 3.9 years from 22 semi-captive female Asian elephants in Thai elephant camps to characterize LH and progestin patterns throughout the e...