The most common way to produce bio-ethanol is fermenting raw materials using suitable microorganism. For this fermentation process to produce ethanol from Artocarpus Heterophyllus which is generally known as jack fruit, Saccharomyces Cerevisiae is used as a microorganism. Saccharomyces is used to produce high yield of ethanol due to its naturally adopting property and its high tolerance rate of...

Negative environmental consequences of fossil fuels and concerns about petroleum supplies have spurred the search for renewable transportation biofuels. To be a viable alternative, a biofuel should provide a net energy gain, have environmental benefits, be economically competitive, and be producible in large quantities without reducing food supplies. We use these criteria to evaluate, through l...

The stress imposed by ethanol to Saccharomyces cerevisiae cells are one of the most challenging limiting factors in industrial fuel production. Consequently, toxicity and tolerance high concentrations has been subject extensive research, allowing identification several genes important for increasing this factor. However, studies were performed with well-characterized laboratory strains, how res...

A recombinant strain of Escherichia coli FBWHR was used for ethanol fermentation from hot-water sugar maple wood extract hydrolyzate in batch experiments. Kinetic studies of cell growth, sugar utilization and ethanol production were investigated at different initial total sugar concentrations of wood extract hydrolyzate. The highest ethanol concentration of 24.05 g/L was obtained using an initi...

Flow cytometry was used to evaluate the effect of initial ethanol concentrations on cyanobacterial strains of Synechocystis PCC 6803 [wild-type (WT), and ethanol producing recombinants (UL 004 and UL 030)] in batch cultures. Ethanol recombinants, containing one or two metabolically engineered cassettes, were designed towards the development of an economically competitive process for the direct ...

Understanding ethanol-induced stresses and responses in biofuel-producing bacteria at systems level has significant implications in engineering more efficient biofuel producers. We present a computational study of transcriptomic and genomic data of both ethanol-stressed and ethanol-adapted E. coli cells with computationally predicated ethanol-binding proteins and experimentally identified ethan...

The physiological role and possible functional substitution of each of the five alcohol dehydrogenase (Adh) isozymes in Saccharomyces cerevisiae were investigated in five quadruple deletion mutants designated strains Q1-Q5, with the number indicating the sole intact ADH gene. Their growth in aerobic batch cultures was characterised in terms of kinetic and stoichiometric parameters. Cultivation ...

BACKGROUND Oxidative stress due to excessive production of reactive oxygen species (ROS) and subsequent lipid peroxidation plays a critical role in renal ischemia/reperfusion (IR) injury. The purpose of current study is to demonstrate the effect of antecedent ethanol exposure on IR-induced renal injury by modulation of oxidative stress. MATERIALS AND METHODS Bilateral renal warm IR was induce...

The objective of this project is to develop a process for the parallel production of ethanol and cellulose in a pulp mill, by applying a prehydrolysis step to softwood chips to extract hexose sugars for their subsequent fermentation in ethanol. Two kinds of pretreatments were compared, an acid hydrolysis and a two-step treatment composed of an autohydrolysis and a post-hydrolysis of the resulti...

The technical and economical feasibilities of a novel integrated biomass gasification and fuel cell combined heat and power (CHP) system were analyzed for supplying heat and power in an ethanol plant from distillers grains (DG) and corn stover. In a current dry-grind plant with an annual production capacity of 189 million liters (50 million gallons) of ethanol, the energy cost for ethanol produ...