We have developed a multiplex fluorophore molecular beacon () with fluorophores located at its end to produce unique FRET (Fluorescence Resonance Energy Transfer). It exhibited diverse fluorescence properties depending on the mixing pattern, such as large Stokes shift emission and multiple colors, namely, blue, green and red using one excitation wavelength. Our also worked in probing a target p...

The use of absorption and fluorescence spectroscopy for following the progress of the photoreaction between DNA and 4-aminomethyl-4,5‘,8-trimethylpsoralen (AMT) has been investigated. Absorption at long wavelengths and fluorescence both decline upon intercalation of AMT into the DNA helix. The loss of fluorescence from AMT and the accompanying appearance of monoadduct fluorescence upon irradiat...

Adsorption of polymers onto particles has been a subject of study because of its importance in the behavior of suspensions.1,2 To understand the adsorption/desorption process in detail, studies have been carried out with model systems. Some studies have employed polystyrene (PS) adsorbed onto metals, silica, glass, and mica.3-7 In previous work, we reported on the use of pyrene-labeled PS to st...

   Particles with the size of 1-100 nm are known as nanoparticles (NPs). The widespread use of silver NPs (AgNPs) makes it familiar in different industries. They have unique properties as a result of their high surface to volume ratio, although aggregation of NPs interferes with their functions. This phenomenon has several side effects on the environment, the amount of which may depend on t...

Objective(s):Cefixime (Cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. There are some procedures for the determination of Cfx in pharmaceutical formulations and biological samples. Herein a spectrofluorimetric method was proposed for Cfx determination based on the fluorescence quenching of terbium-danofloxa...

Fluorescence imaging has been demonstrated to be able to detect and diagnose different tumour types in a range of biological tissues including cervix, lung, and along the gastrointestinal tract. An approach based on laser induced tissue autofluorescence may be applied minimally invasively using endoscopes, although there is a high degree of overlap in endogenous fluorophore excitation/emission ...

a flow injection system is reported for the determination of fluoride based on its effects on the fluorescence intensity of al-morin complex at ph 3.0 in ethanol-warn mixture. the effect of important parameters such as excitation and emission wavelengths, ph, reagents concenkations, flow rate of the carrier stream, sample injection volume, time of measurepent and interferences are reported. cat...

Single-molecule fluorescence imaging is at the forefront of tools applied to study biomolecular dynamics both in vitro and in vivo. The ability of the single-molecule fluorescence microscope to conduct simultaneous multi-color excitation and detection is a key experimental feature that is under continuous development. In this paper, we describe in detail the design and the construction of a sop...

In green-sulfur bacteria sunlight is absorbed by antenna structures termed chlorosomes, and transferred to the RC via the Fenna-Matthews-Olson (FMO) complex. FMO consists of three monomers arranged in C3 symmetry where each monomer accommodates eight Bacteriochlorophyll a (BChl a) molecules. It was the first pigment-protein complex for which the structure has been determined with high resolutio...

We describe the design, properties, and performance of an excitation-emission (EE) fluorimeter that enables spectral characterization of an object simultaneously with respect to both its excitation and its emission properties. Such devices require two wavelength-selecting elements, one in the optical path of the excitation broadband light to obtain tunable excitation and the other to analyze th...