The regulation by protein kinase C (PKC) of recombinant voltage-gated potassium (K) channels in frog oocytes was studied. Phorbol 12-myristate 13-acetate (PMA; 500 nM), an activator of PKC, caused persistent and large (up to 90%) inhibition of mouse, rat, and fly Shaker K currents. K current inhibition by PMA was blocked by inhibitors of PKC, and inhibition was not observed in control experimen...

Small (3.5 1) laboratory microcosms containing water, sediment and Elodea canadensis were treated with the herbicides diquat and terbutryne. After treatment total numbers of planktonic bacteria increased significantly by 3-1 1 -fold and heterotroph counts also rose significantly, by 3-23-fold but the population increases of exoenzyme-producing bacteria (3-1 13-fold) were not always significant....

Thattekad bird sanctuary, located in the Western Ghats of Kerala, India, which hosts an unexplored microbial community, is selected for present investigation. Microbes play a major role mineral recycling and nutrient absorption by flora fauna habitat. Various bacterial extracellular enzymes facilitate all these activities. The increasing demand favor green technology encouraged us to focus on e...

Activated sludge used for wastewater treatment globally is composed of a high-density microbial community of great biotechnological significance. In this study the presence and purpose of quorum sensing via N-acylated-l-homoserine lactones (AHLs) in activated sludge was explored. The presence of N-heptanoyl-l-homoserine lactone in organic extracts of sludge was demonstrated along with activatio...

PURPOSE The goal of this study was to investigate the role of the small guanosine triphosphatase (GTPase), Rho, in the corneal epithelial response to extracellular matrix (ECM) molecules. The avian corneal epithelial model was used to establish that Rho is required for actin reorganization and tyrosine phosphorylation of integrin-mediated signal pathway proteins. METHODS Whole embryonic corne...

The bacterium strain No. 12 isolated from a phenol-adapted activated sludge as a floc-forming bacterium was found to produce an exoenzyme which could deflocculate its own floc. As the floc of this bacterium was not deflocculated with EDTA and various enzymes such as cellulase, protease, and lysozyme, this exoenzyme was isolated and purified. The optimal pH of the enzyme for deflocculation was 3...

Bartonella bacilliformis was continuously internalized into human endothelial cells beginning shortly after addition of the bacteria and continuing for at least 24 h after infection in vitro, with a major increase in uptake occurring between 16 and 24 h. Preincubation of endothelial cells with C3 exoenzyme, which inactivated intracellular Rho-GTPase, blocked internalization of the bacteria. Add...